pdf tender document information - CCMB

Acquire CD spectra in continuous temperature ramp mode for determining thermodynamic data
(simultaneous analysis of T melt curves at each wavelength, to observe the secondary/tertiary structural
changes at each thermal transition, record Tmelt curves at fewer wavelengths in a single temperature
ramp experiment.
Acquire CD spectra as a function of temperature in a stepped temperature ramp mode i.e CD vs Wavelength
vs Temperature data.
Acquiring CD signal in a continuous temperature ramp mode for determining thermodynamic data at
single wavelength (Tmelt technique for determining protein stability)
Post acquisition smoothing of the CD spectra would be preferred to smoothing during acquisition so that raw data is
never lost.
Should be able to export data to other file formats like text files etc.
System should include a standalone PC with the latest Operating system and standard communication interface and
control.
The offered system should include the following software along with the required licenses (min imum of 5 user
licenses)
i. The basic control & analysis software should be capable of full system control monochromator settings, post
acquisition smoothing, export to spreadsheet and other file formats, kinetic data acquisition, multiwavelength
variable temperature programming, denatured protein analysis, curve fitting etc.
ii.
iii.
Secondary structure analysis program to be included
Program for analysis of multiwavelength temperature scan data capable of calculating midpoint temperatures,
Van’t Hoff enthalpies of thermal transitions, calculate concentrations against temperature, providing
thermodynamic and structural information etc.
System should be flexible and allow field upgrades for different applications and measurement modes like Peltier
temperature control, Simultaneous fluorescence detection, Stopped flow CD etc
Suitable Nitrogen gas flow meter for purging the optics and the sample compartment should be included with the
system.
3) Accessories to be offered optionally
i. Peltier temperature control accessory for CD.
ii. Peltier temperature control accessory for CD, simultaneous Fluorescence and fluorescence detected CD)
Items {3 (i) and 3 (ii)} should have the following specifications and features:
Temperature range: -10 deg to 100deg C
Temperature accuracy: ± 0.05 deg C or better
Should have cuvette temperature probe for monitoring sample temperature
Stirring facility to be included
Should be able to accommodate both the rectangular and cylindrical cuvettes of different pathlengths.
Hardware control of the Peltier should be through the control software of the CD instrument.
Step wise and continuous temperature ramping facility.
Additional detectors, suitable recirculating cooler (water bath) and other accessories needed for the above
Peltier units {Items 3 (i) and 3 (ii)} to be included in the offer.
iii.
iv.
Accessory for stopped flow with peltier temperature control, Four cell auto changer with Peltier temperature
control, Fluorescence polarization and anisotropy accessory, Diffuse reflectance accessory to be offered.
Cells of different pathlength 0.01mm, 0.02 mm, 0.05 mm (rectangular cells); 0.1 mm,0.2mm, 0.5mm, 1mm,
2mm, 5mm & 10 mm ( rectangular & cylindrical cells) -- 4 each
v. Suitable adapters / cell holders for the above to be offered.
vi. Spare lamps (2 nos )
General clauses
1. Main system including subsystems, computers and accessories should operate on 220V, 50 Hz mains power.
BID DOCUMENT (YC)
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