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ITEM NO.10 – SPECTRAL CONFOCAL MICROSCOPE, QTY: 1 NO. (FILE REF:
34/CCMB/APP/407/P12)
SPECTRAL CONFOCAL MICROSCOPE FOR HIGH SENSITIVE AND HIGH RESOLUTION IMAGING OF
BIOLOGICAL SAMPLES:
The system should be of latest state of the art technology capable of high sensitive spectral confocal imaging of fixed
& Live Biological samples. The system should include multichannel Fluorescence imaging with Z-stack, time-lapse, co
localization, FRET, FRAP, photon counting, Photo activation and conversion.
The system should consist of:
1. Fully Motorized Inverted Fluorescence Research Microscope for BF/DIC/FL with dedicated TFT/LCD display for
full control of Microscope. Motorized XY Scanning stage with universal sample holders for slides, Petri dish and
other live cell sample holders like 6 well plate, 96 well plate for tile, mosaic and multipoint imaging. Motorized 6
position DIC nosepiece, motorized BF/PH/DIC Condenser, 6 position motorized FL turret. High precision built in Z-
focus drive with step resolution of 15nm or better. Transmitted and Reflected Light illumination with 100W halogen
and 120W metal halide with PC control and long lifetime of 2000 hours. High resolution confocal grade Plan
apochromat objectives corrected for both UV & VIS lines 10x/0.45, 20x/0.70,40x/0.85,40x/1.30oil, 60/63x/1.40oil,
100x/1.4 oil immersion along with DIC accessories for all objectives. Bandpass fluorescent filters for DAPI, GFP,
FITC, Cy3 and Cy5.
2. Anti vibration table.
3. Completely motorized Point scanning laser based Confocal unit with built in Internal high sensitive spectral
detectors (Ga AsP/HyD) based imaging for at least 2 channels in addition to the sta ndard 2 channel PMT
detection with independent gain controls for all channels. All the FL detectors of the scan head should be filter free
with freely selectable emission band width detection capability to suit to the emission spectra of the dyes. All
detectors should be spectral. The detection unit should be inbuilt in the scanner unit. The system should include
simultaneous detection and separation of at least 5 fluorophores based on spectral detectors. High sensitive
motorized dichroic system with low angle of incidence with high efficient optics.
The system should be capable of recording emission spectra with minimum spectral resolution 5nm or better.
Computer controlled continuously variable confocal pinhole with software control. Scan resolution of 6Kx6K or
above. Frame rates of 7 fps or higher with spectral detectors @512x512 pixels in spectral detection mode. Real
ROI scan and bleach with various ROI shapes should be possible for FRAP experiments. The scan field diagonal
should be 20 mm F.O.V. or more.
4. PMT based transmitted light detector for DIC imaging.
5. Laser unit with control electronics including multiline Ar 458/488/514nm, DPSS 561nm, HeNe 633nm & BDL
405/408nm, all laser lines including 405nm should be controlled through 8 channel AOTF for fast laser switching
and attenuation. Seperate laser ports for UV/405nm, Vis, IR and additional port for future upgrades is essential.
System should have capability to upgrade for tunable laser with visible wavelength.
6. Latest branded 64 bit control computer with Intel Xeon 6 Core Processor, DDR RAM 8 GB HDD: 1 TB SATA,
DVD, Super Multi SATA +R/RW, Graphics : AT Fire GL V5200 256MB DH DVI, Gigabit Ethernet, Win 7 Ultimate
64 bit OS , USB 2.0, Fire wire. Large 30” LCD/LED/ TFT monitor.
7. Confocal system control software capable of controlling all motorized functions of microscope, scan head, lasers,
AOTF including image acquisition & processing. Image acquisition for 3D, 4D, on-line spectral Imaging based on
lambda stacks. Time series, FRAP, FRET, Photo activation and conversion. Imaging without bleed through and
autofluorescence separation by online emission fingerprinting technique. Advanced multidimensional software for
3D analysis with Deconvolution, multichannel volume rendering, reconstruction, measurements across z stack,
movie, co-localization with histogram analysis, intensity profiles for quantification etc....
Optional items:
1) Additional HeNe 594nm laser should be offered.
2) Upgradable to FCS/FCCS unit for Single Molecule Detection: The FCS unit with APD detectors should be capable
of performing auto and cross correlation measurements in live cells and solutions for a wide range of dyes and proteins.
3) The FCS unit should have facility for elimination/suppression of other excitation laser lines. All laser lines for Confocal
Imaging should be capable of working in FCS/FCCS mode. Dedicated Plan Apochromat 40x/1.20 or 63x/1.2 N.A.
water should be offered along with the system. FCS measurement software for Auto and Cross correlation
capabilities to be quoted.
4) Any patented detection system which is more sensitive and multi user friendly may also be offered.
5) Additional GaAsP/HyD detector to be offered.
BID DOCUMENT (YC)
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