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Optional items: 1. Ergo Binocular Tube 2. The microscope with computer controlled IR LED/Laser based focus drift compensation mechanism for Z movement to get correct focus during long term time lapse imaging experiments. 3. Programmable and computer controlled incubation system for full automation. Compact incubator with CO2 controller and heating insert for slides, petridishes, 6well and 96 well plates should be quoted. Co2 controller and Temperature control unit should be controlled using the software. 4. Spare Lamps for Metal halide 5. Spare lamps for transmission light 6. 60x and 100x phase objectives, Immersion Oil Other Important clauses: System should work with 220V@50Hz Complete system should carry one year onsite comprehensive warranty. The computer should carry three years onsite comprehensive warranty. Complete Service and technical manuals with detailed documentation, user manual should be supplied along with the system. List of users of similar research institutions in India to be provided with addresses and contact details. Technical presentation & demonstration of similar system in other research institutes / centers / universities should be arranged on request. Criteria for selection would be based not only on specifications, features, clauses and conditions but on proven performance of Confocal microscope and technical support. Therefore all the points mentioned in the tender should be answered. BID DOCUMENT (YC) Page 42 of 63
ITEM NO.10 – SPECTRAL CONFOCAL MICROSCOPE, QTY: 1 NO. (FILE REF: 34/CCMB/APP/407/P12) SPECTRAL CONFOCAL MICROSCOPE FOR HIGH SENSITIVE AND HIGH RESOLUTION IMAGING OF BIOLOGICAL SAMPLES: The system should be of latest state of the art technology capable of high sensitive spectral confocal imaging of fixed & Live Biological samples. The system should include multichannel Fluorescence imaging with Z-stack, time-lapse, co localization, FRET, FRAP, photon counting, Photo activation and conversion. The system should consist of: 1. Fully Motorized Inverted Fluorescence Research Microscope for BF/DIC/FL with dedicated TFT/LCD display for full control of Microscope. Motorized XY Scanning stage with universal sample holders for slides, Petri dish and other live cell sample holders like 6 well plate, 96 well plate for tile, mosaic and multipoint imaging. Motorized 6 position DIC nosepiece, motorized BF/PH/DIC Condenser, 6 position motorized FL turret. High precision built in Z- focus drive with step resolution of 15nm or better. Transmitted and Reflected Light illumination with 100W halogen and 120W metal halide with PC control and long lifetime of 2000 hours. High resolution confocal grade Plan apochromat objectives corrected for both UV & VIS lines 10x/0.45, 20x/0.70,40x/0.85,40x/1.30oil, 60/63x/1.40oil, 100x/1.4 oil immersion along with DIC accessories for all objectives. Bandpass fluorescent filters for DAPI, GFP, FITC, Cy3 and Cy5. 2. Anti vibration table. 3. Completely motorized Point scanning laser based Confocal unit with built in Internal high sensitive spectral detectors (Ga AsP/HyD) based imaging for at least 2 channels in addition to the sta ndard 2 channel PMT detection with independent gain controls for all channels. All the FL detectors of the scan head should be filter free with freely selectable emission band width detection capability to suit to the emission spectra of the dyes. All detectors should be spectral. The detection unit should be inbuilt in the scanner unit. The system should include simultaneous detection and separation of at least 5 fluorophores based on spectral detectors. High sensitive motorized dichroic system with low angle of incidence with high efficient optics. The system should be capable of recording emission spectra with minimum spectral resolution 5nm or better. Computer controlled continuously variable confocal pinhole with software control. Scan resolution of 6Kx6K or above. Frame rates of 7 fps or higher with spectral detectors @512x512 pixels in spectral detection mode. Real ROI scan and bleach with various ROI shapes should be possible for FRAP experiments. The scan field diagonal should be 20 mm F.O.V. or more. 4. PMT based transmitted light detector for DIC imaging. 5. Laser unit with control electronics including multiline Ar 458/488/514nm, DPSS 561nm, HeNe 633nm & BDL 405/408nm, all laser lines including 405nm should be controlled through 8 channel AOTF for fast laser switching and attenuation. Seperate laser ports for UV/405nm, Vis, IR and additional port for future upgrades is essential. System should have capability to upgrade for tunable laser with visible wavelength. 6. Latest branded 64 bit control computer with Intel Xeon 6 Core Processor, DDR RAM 8 GB HDD: 1 TB SATA, DVD, Super Multi SATA +R/RW, Graphics : AT Fire GL V5200 256MB DH DVI, Gigabit Ethernet, Win 7 Ultimate 64 bit OS , USB 2.0, Fire wire. Large 30” LCD/LED/ TFT monitor. 7. Confocal system control software capable of controlling all motorized functions of microscope, scan head, lasers, AOTF including image acquisition & processing. Image acquisition for 3D, 4D, on-line spectral Imaging based on lambda stacks. Time series, FRAP, FRET, Photo activation and conversion. Imaging without bleed through and autofluorescence separation by online emission fingerprinting technique. Advanced multidimensional software for 3D analysis with Deconvolution, multichannel volume rendering, reconstruction, measurements across z stack, movie, co-localization with histogram analysis, intensity profiles for quantification etc.... Optional items: 1) Additional HeNe 594nm laser should be offered. 2) Upgradable to FCS/FCCS unit for Single Molecule Detection: The FCS unit with APD detectors should be capable of performing auto and cross correlation measurements in live cells and solutions for a wide range of dyes and proteins. 3) The FCS unit should have facility for elimination/suppression of other excitation laser lines. All laser lines for Confocal Imaging should be capable of working in FCS/FCCS mode. Dedicated Plan Apochromat 40x/1.20 or 63x/1.2 N.A. water should be offered along with the system. FCS measurement software for Auto and Cross correlation capabilities to be quoted. 4) Any patented detection system which is more sensitive and multi user friendly may also be offered. 5) Additional GaAsP/HyD detector to be offered. BID DOCUMENT (YC) Page 43 of 63
Page 1 and 2: CENTRE FOR CELLULAR & MOLECULAR BIO
Page 3 and 4: VERY IMPORTANT: Pre-Bid Conference
Page 5 and 6: CHAPTER - II: INSTRUCTION TO BIDDER
Page 7 and 8: 10 Documents Establishing Bidder’
Page 9 and 10: E. OPENING AND EVALUATION OF BIDS T
Page 11 and 12: CHAPTER - III: GENERAL CONDITIONS O
Page 13 and 14: 15.2This warranty shall remain vali
Page 15 and 16: CHAPTER - IV: SPECIAL CONDITIONS OF
Page 17 and 18: 17 Supplier Integrity 17.1The Suppl
Page 19 and 20: CHAPTER - VI: BID SECURITY FORM Whe
Page 21 and 22: CHAPTER - VIII: BIDDER’S PERFORMA
Page 23 and 24: CHAPTER - X: DEVIATION STATEMENT FO
Page 25 and 26: CHAPTER - XII: CONTRACT FORM THIS A
Page 27 and 28: CHAPTER XIV: QUALIFICATION REQUIREM
Page 29 and 30: ITEM NO.2 - FLUORESCENCE MICROSCOPE
Page 31 and 32: 3. Ambient temperature: 42 deg. C 4
Page 33 and 34: ITEM NO.5 - HIGH RESOLUTION FTIR SY
Page 35 and 36: ITEM NO.6 - PROTEIN PURIFICATION SY
Page 37 and 38: ITEM NO.7 - PHOSPHOR IMAGER ALONG W
Page 39 and 40: ITEM NO.8 - UPRIGHT FLUORESCENCE MI
Page 41: ITEM NO.9 - ADVANCED MOTORIZED INVE
Page 45 and 46: ITEM NO.11 - HIGH RESOLUTION MASS S
Page 47 and 48: ITEM NO.12 - HIGH SENSITIVITY CD SP
Page 49 and 50: 2. All the above mentioned technica
Page 51 and 52: . The computer and printer offered
Page 53 and 54: 2. Cost should include three years
Page 55 and 56: 2. Capacity : 8 x 5 ml or more Maxi
Page 57 and 58: ITEM NO.17 - ZEBRA FISH IMAGING AND
Page 59 and 60: ITEM NO.18 - HIGH CONTENT SCREENING
Page 61 and 62: 7. Software supplied should be the
Page 63: Main system including subsystems, c

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