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HERBICIDES in Asian rice - IRRI books - International Rice ...

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Test 2. The efficacy of 38 <strong>rice</strong> accessions or germplasm l<strong>in</strong>es identified as allelopathic<br />

for aquatic weeds <strong>in</strong> dry-seeded and water-seeded culture was evaluated <strong>in</strong> the<br />

field, <strong>in</strong> 1990 and 1991 for dry-seeded <strong>rice</strong> and <strong>in</strong> 1992 and 1993 for water-seeded<br />

<strong>rice</strong>. Four cultivars (Gulfmont, Mercury, Palmyra, and Rexmont) that have no apparent<br />

allelopathic activity were the controls.<br />

The dry-seeded cultivars were seeded <strong>in</strong> 2.1- × 1.1-m plots, <strong>in</strong> three rows spaced<br />

53 cm apart, with three replications. Unwanted weeds were controlled with herbicides<br />

(propanil, fenoxaprop, and bentazon). Aquatic weeds, <strong>in</strong>clud<strong>in</strong>g ducksalad, purple<br />

ammannia ( Ammannia cocc<strong>in</strong>ea ), and disc waterhyssop ( Bacopa rotundifolia ) that<br />

germ<strong>in</strong>ated after the plots were flooded were not affected by the herbicides.<br />

When the <strong>rice</strong> was ready for harvest, the 53-cm-wide space on each side of the<br />

center row was evaluated for weed suppression. Weeds between the outside <strong>rice</strong> rows<br />

were pulled, dried, and weighed. In 1990, a core soil sample (6.5 cm diameter by 2.5<br />

cm deep) was taken on each side of the center row of eight test accessions and three<br />

checks to determ<strong>in</strong>e root biomass. Soil samples were washed through a 20-mesh screen,<br />

and the roots dried and weighed.<br />

In the wet-seeded experiments, seeds of the same 38 accessions and four check<br />

cultivars were placed <strong>in</strong> cloth bags, soaked <strong>in</strong> water for 24 h, and dra<strong>in</strong>ed. The soaked<br />

seeds were water-seeded <strong>in</strong> 2.1- × 1.1 -m plots <strong>in</strong> three 2-cm-deep furrows spaced<br />

53 cm apart. Plots were surrounded with metal levees to prevent movement of the <strong>rice</strong><br />

seedl<strong>in</strong>gs.<br />

Aquatic weeds ducksalad, purple ammannia, and disc waterhyssop germ<strong>in</strong>ated<br />

when the <strong>rice</strong> germ<strong>in</strong>ated. Unwanted weeds were controlled by hand weed<strong>in</strong>g. Visual<br />

rat<strong>in</strong>gs were made 7 wk after flood<strong>in</strong>g. At <strong>rice</strong> maturity, weeds between the outside<br />

<strong>rice</strong> rows were harvested, dried, and weighed.<br />

Test 3. In 1992-93, we compared the allelopathic activity of <strong>rice</strong> <strong>in</strong> dry- and<br />

water-seeded cultures, seeded <strong>in</strong> rows or broadcast. Five allelopathic <strong>rice</strong> l<strong>in</strong>es-T65/<br />

2 × TN1, IR644-1-63-1-1, IR782- 131, IR643-75-1-1, and IR788-16-1-1-1 and<br />

Rexmont, a standard Arkansas cultivar without allelopathic activity, were evaluated<br />

<strong>in</strong> a split-split plot design with four replications. Dry- and water-seeded cultures were<br />

ma<strong>in</strong> plots, broadcast and row seed<strong>in</strong>g were subplots, and <strong>rice</strong> l<strong>in</strong>es were subsubplots<br />

(2 × 1 m). Rows were spaced 53 cm apart.<br />

In the dry-seeded culture, <strong>rice</strong> seeds were broadcast on the soil surface or seeded<br />

<strong>in</strong> 2.5-cm-deep furrows and covered with soil. In the water-seeded culture, <strong>rice</strong> seed<br />

pregerm<strong>in</strong>ated for 36 h was broadcast or row-seeded onto soil flooded 5 cm deep. All<br />

water-seeded plots were surrounded by a metal levee to prevent movement of <strong>rice</strong><br />

seedl<strong>in</strong>gs. A bird nett<strong>in</strong>g protected the plots from immediately after seed<strong>in</strong>g until the<br />

<strong>rice</strong> plants were 15 cm tall.<br />

In dry-seeded <strong>rice</strong>, aquatic weeds germ<strong>in</strong>ated 37 d after seed<strong>in</strong>g (7 d after flood<strong>in</strong>g);<br />

<strong>in</strong> water-seeded <strong>rice</strong>, 5-7 d after seed<strong>in</strong>g. In dry-seeded <strong>rice</strong>, unwanted weeds<br />

were controlled by propanil, bentazon, and fenoxaprop; <strong>in</strong> water-seeded <strong>rice</strong>, by hand<br />

weed<strong>in</strong>g. Aquatic weeds <strong>in</strong>cluded ducksalad, purple ammannia, and disc waterhyssop.<br />

Identify<strong>in</strong>g allelopathy <strong>in</strong> <strong>rice</strong> germplasm 221

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