Tissue Banking Overview: Washington University Medical Center

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8/21/2012 Specimen Collection • Dedicated personnel on call to collect biospecimens from BJH OR, surgical pathology suite, and clinics. • Self-contained biospecimen procurement kits and procurement tutorials to collect off-site. • Collection of surgically resected tissue, peripheral blood, bone marrow, needle biopsies, and lavage fluid. Specimen Preservation • Snap Freezing Difficult / Requires resources Poor histology Expensive storage Best molecular yield • Ethanol Fixation / LMPE Difficult / Requires resources Stability issues Good histology Cheap storage Good molecular yield • “RNALater” Still need to freeze / embed Poor histology Convenient Good nucleic acid yield • Formalin Fixation / PE Poor molecular yield Standardized Stable Excellent histology 14
8/21/2012 Specimen Storage • Ultralow mechanical freezers (-80°C) Cheaper (Long Term) Error Prone Less Stable • Liquid nitrogen (-130°C) More Stable Less Error Prone More Expensive • Vacuum sealed (4°C) Paraffin Blocks / Slides No Data Gross procurement of tissues for research banking ‣ Academic hospitals with research tissue repositories often derive many internal specimen acquisitions from their site's surgical pathology service ‣ Typically, such acquisitions come from appropriately consented tissue discards sampled from surgical resections ‣Tissue, if not banked, would otherwise be thrown away since it is not needed for clinical diagnosis ‣ Surgical pathology has patient care as its primary mission, so competing needs for tissue inevitable arise: preserving adequate tissue for clinical diagnosis is key ‣ Involvement of trained pathology personnel is important McDonald SA, Chernock RD, Leach TA, Kahn AA, Yip JH, Rossi J, Pfeifer JD. Procurement of human tissues for research banking in the surgical pathology laboratory: prioritization practices at Washington University Medical Center. In press, Biopreservation and Biobanking, 2011. 15
Page 1 and 2: 8/21/2012 ICTS Brown Bag Seminar
Page 3 and 4: 8/21/2012 Washington University Med
Page 5 and 6: 8/21/2012 BJCIH Laboratory for Tran
Page 7 and 8: 8/21/2012 Classification of banked
Page 9 and 10: 8/21/2012 Specimen Collection • D
Page 11 and 12: 8/21/2012 Advantages of online syst
Page 13: 8/21/2012 Specimen QA • Specimen
Page 17 and 18: 8/21/2012 Tissue banking principles
Page 19 and 20: 8/21/2012 Acknowledgments • Sitem
Page 21 and 22: 8/21/2012 WUMC Biobanking 2011 Regu
Page 23 and 24: 8/21/2012 Contraindications to tiss
Page 25 and 26: 8/21/2012 Organ-specific contraindi
Page 27 and 28: 8/21/2012 “Adjacent normal” tis
Page 29 and 30: 8/21/2012 Tissue Microarrays Advant
Page 31 and 32: 8/21/2012 Operating Procedures for
Page 33 and 34: 8/21/2012 General procedure ‣ Use
Page 35 and 36: 8/21/2012 caTissue: Search Results
Page 37: 8/21/2012 Comparison between umbili

Tissue Banking Overview: Washington University Medical Center

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