Tissue Banking Overview: Washington University Medical Center
Tissue Banking Overview: Washington University Medical Center
Tissue Banking Overview: Washington University Medical Center
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8/21/2012<br />
<strong>Washington</strong> <strong>University</strong><br />
<strong>Medical</strong> <strong>Center</strong><br />
Biorepository<br />
• A centralized resource for biospecimens and laboratory<br />
procedures, started in 1997 as effort with Siteman Cancer Ctr.<br />
• Laboratory for Translational Pathology /<strong>Tissue</strong> Procurement<br />
Core) , 14 FTEs<br />
• ~500,000 samples (tissues and biofluids) derived from a<br />
variety of internal and external collection protocols (~70 open<br />
currently) and clinical trials; traditional focus is neoplasia<br />
• Collection, storage, quality assurance and scientific<br />
utilization/impact of biospecimens, to better understand the<br />
molecular / genomic basis of cancer and translations to pt. care<br />
• Fee-for-service mechanism, usage on a request-driven basis<br />
• Resource limited currently<br />
Wash U Biorepository Workflow<br />
Incoming<br />
tissue,<br />
blood ,<br />
and other<br />
biofluids<br />
SAMPLE ACCESSION,<br />
PREP & PROCESSING<br />
Some samples<br />
Most samples<br />
Some samples<br />
Investigator<br />
requests<br />
(samples<br />
pulled from<br />
storage in<br />
response)<br />
STORAGE<br />
(mainly frozen<br />
biospecimens, few<br />
paraffin blocks)<br />
HISTOLOGY<br />
Some samples<br />
Sections and/or<br />
fresh-frozen<br />
tissues<br />
disbursed to<br />
investigators<br />
Samples disbursed to<br />
investigators; however, for<br />
most samples, histology<br />
or molecular workup is<br />
needed first (black arrows)<br />
MOLECULAR<br />
Make RNA & DNA<br />
Sample flow within repository<br />
Sample Entry Points<br />
Sample Exit Points<br />
Key component of repository<br />
Molecular samples disbursed to<br />
investigators (following isolation<br />
and QA)<br />
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