Tissue Banking Overview: Washington University Medical Center

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8/21/2012 Fee for service schedule • In an effort to better meet its operational expenses, the Tissue Procurement Core has initiated a fee-for-service schedule • Labor, reagents/consumables, and/or infrastructure components underlie the charges • Fee-for-service schedule is shared with investigators when they contact the bank for protocol initiation or sample withdrawal • Examples of charges: Service Unit Actual Cost Ficoll Processing 10 ml peripheral blood or 2 ml BM or 10^7 cells $76.80 Ficoll Processing (large scale) 60 ml peripheral blood or 12 ml BM or 10^8 cells $101.78 CPT Tube Processing and Cell Freezing per tube $31.97 Cell Cryoperservation per 10^7 cells $4.28 Cell Processing and Cryopreservation 10‐20ml peripheral blood or 2‐5ml of BM $31.67 Laser capture microscope tech time per hour $59.95 Laser capture microscope use per hour $29.95 DNA prep and QA from cell pellet per sample $17.29 Tissue Fixation and Embedding $15.91 Storage under liquid N2 vapor per stored aliquot per year $0.21 Tissue banking and clinical trials • Is important for tissue banking, especially for pharmacogenomics and correlative biomarker studies Objectives: • relate biomarkers to onset, course, and outcome of disease and the level of response to various treatments • predict who will respond to therapy • modification of expression of targets/other biomarkers in response to therapy • future use of tissues for hypotheses not known at present (depending on consent scope-of-use) 8
8/21/2012 Specimen Collection • Diseased Tissue Genetic, biochemical, or histological analysis of disease process Sampling Site Fixed vs. Frozen (Dictates storage needs and future utilization) Fixation Type Embedding Medium • Serum / Plasma Biochemical studies Aliquot and freeze • Peripheral Leukocytes/Bone Marrow Genomic (germ line) analysis; Occult tumor cell detection Whole blood Enriched nucleated cell fraction Density gradient centrifugation Cryopreservation / Immortalization • CSF and other fluids Cytospin Freeze Terminology for collected/ banked samples Identified – Labeled with personal identifiers such as name or SSN Coded – Labeled with a clinical trial subject number, investigator has access to the code De-identified – Labeled with a unique second number; link between this second number and the clinical trial subject number is maintained in a highly secure database, and generally unavailable to investigators and patients Anonymized – Link described above is irreversibly removed, so that specimens cannot be traced back to patient identities by anyone Anonymous – Samples without personal identifiers, and whose identity is unknown. An increasingly nebulous concept these days, with the advent of whole exome and genome sequencing, where the data is increasingly viewed as essentially the patient’s identity 9
Page 1 and 2: 8/21/2012 ICTS Brown Bag Seminar
Page 3 and 4: 8/21/2012 Washington University Med
Page 5 and 6: 8/21/2012 BJCIH Laboratory for Tran
Page 7: 8/21/2012 Classification of banked
Page 11 and 12: 8/21/2012 Advantages of online syst
Page 13 and 14: 8/21/2012 Specimen QA • Specimen
Page 15 and 16: 8/21/2012 Specimen Storage • Ultr
Page 17 and 18: 8/21/2012 Tissue banking principles
Page 19 and 20: 8/21/2012 Acknowledgments • Sitem
Page 21 and 22: 8/21/2012 WUMC Biobanking 2011 Regu
Page 23 and 24: 8/21/2012 Contraindications to tiss
Page 25 and 26: 8/21/2012 Organ-specific contraindi
Page 27 and 28: 8/21/2012 “Adjacent normal” tis
Page 29 and 30: 8/21/2012 Tissue Microarrays Advant
Page 31 and 32: 8/21/2012 Operating Procedures for
Page 33 and 34: 8/21/2012 General procedure ‣ Use
Page 35 and 36: 8/21/2012 caTissue: Search Results
Page 37: 8/21/2012 Comparison between umbili

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