Tissue Banking Overview: Washington University Medical Center

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8/21/2012 Sampling issues This sample of metastatic adenocarcinoma in the liver is largely necrotic. Therefore, this would not be an ideal specimen for most studies, especially digestion of the tissue for RNA expression studies (H&E, 200x) The endometrial glands of this uterus specimen are of primary interest, but the specimen also contains stromal and vascular cells. Thus, specific isolation of the desired cell type may be needed, for example through laser-capture microscopy, if a digestive assay is planned (H&E, 400x) Contributions of tissue banking Demonstration by IHC that a phosphorylated membrane receptor was overexpressed in human lung carcinoma (brown reaction product) helped support research interest in the receptor (200x) Identification of respiratory epithelium (top) was needed for its isolation by laser capture microscopy, to perform pilot studies for gene expression in diseased lung tissue (H&E, 400x) 26
8/21/2012 “Adjacent normal” tissue This case of metastatic carcinoma to the liver showed inflammatory infiltrates in portal tracts in the adjacent non-neoplastic liver tissue Discrepancies between stated and actual specimen dx: commercial samples • This specimen was billed by a commercial tissue provider as normal lung (H&E, 200x) • This specimen was billed by a commercial tissue provider as normal pancreas (H&E, 200x) 27
Page 1 and 2: 8/21/2012 ICTS Brown Bag Seminar
Page 3 and 4: 8/21/2012 Washington University Med
Page 5 and 6: 8/21/2012 BJCIH Laboratory for Tran
Page 7 and 8: 8/21/2012 Classification of banked
Page 9 and 10: 8/21/2012 Specimen Collection • D
Page 11 and 12: 8/21/2012 Advantages of online syst
Page 13 and 14: 8/21/2012 Specimen QA • Specimen
Page 15 and 16: 8/21/2012 Specimen Storage • Ultr
Page 17 and 18: 8/21/2012 Tissue banking principles
Page 19 and 20: 8/21/2012 Acknowledgments • Sitem
Page 21 and 22: 8/21/2012 WUMC Biobanking 2011 Regu
Page 23 and 24: 8/21/2012 Contraindications to tiss
Page 25: 8/21/2012 Organ-specific contraindi
Page 29 and 30: 8/21/2012 Tissue Microarrays Advant
Page 31 and 32: 8/21/2012 Operating Procedures for
Page 33 and 34: 8/21/2012 General procedure ‣ Use
Page 35 and 36: 8/21/2012 caTissue: Search Results
Page 37: 8/21/2012 Comparison between umbili

Tissue Banking Overview: Washington University Medical Center

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