splice site
splice site
splice site
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• Strictly speaking,self-splicing splicing introns are not<br />
enzymes(catalysts),because they mediate<br />
only one round of RNA processing.<br />
• The chemistry of group II intron splicing and<br />
the RNA intermediates produced are the<br />
same as that of the nuclear pre-mRNA.The<br />
introns uses an A residue within the branch<br />
<strong>site</strong> to attack the phosphodiester bond at the<br />
boundary between its 5’end 5<br />
and the end of<br />
the 5’exon5<br />
exon-that is,at the 5’<strong>splice</strong> 5<br />
<strong>site</strong>.Then it<br />
forms a lariat and is followed by a second<br />
reaction in which the newly freed 3’OH 3<br />
of the<br />
exon attacks the 3’<strong>splice</strong> 3<br />
<strong>site</strong>,releasing the<br />
intron as a lariat and fusing the 3’ 3 and 5’ 5<br />
exons.
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