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Generated by Foxit PDF Creator © Foxit Software http://www.foxitsoftware.com For evaluation only. Seequences at the intron-exon boundary
Generated by Foxit PDF Creator © Foxit Software http://www.foxitsoftware.com For evaluation only. 1.2 The intron is removed in a Form Called a Lariat as the Flanking Exons are joined Two successive transesterification: Step 1: The OH of the conserved A at the branch site attacks the phosphoryl group of the conserved G in the 5’ 5 splice site. As a result, the 5’ 5 exon is released and the 5’-end 5 of the intron forms a three-way junction structure. Step 2: The OH of the 5’ 5 exon attacks the phosphoryl group at the 3’ 3 splice site. As a consequence, the 5’ 5 and 3’ 3 exons are joined and the intron is liberated in the shape of a lariat.
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