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Generated by Foxit PDF Creator © Foxit Software http://www.foxitsoftware.com For evaluation only. The structure of the catalytic region that performs the first transesterificatio n reaction is verh similar in the group2 introns and the premRNA /snRNP complex.
Generated by Foxit PDF Creator © Foxit Software http://www.foxitsoftware.com For evaluation only. 3.4 How does spliceosome find the splice sites reliably Reasons for the recognition errors: (1) The average exon is 150 nt, and the average intron is about 3,000 nt long (some introns are near 800,000 nt).It is quite challenging for the spliceosome to identify the exons within a vast ocean of the intronic sequences. (2) The splice site consensus sequence are rather loose. For example, only AG|G tri-nucleotides is required for the 3’ splice site, and this consensus sequence occurs every 64 nt theoretically. Two kinds of splice-site recognition errors: (1) Splice sites can be skipped. (2) Other sites could be mistakenly recognized.
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