splice site

More documents

Recommendations

Info

Generated by Foxit PDF Creator © Foxit Software http://www.foxitsoftware.com For evaluation only. • Strictly speaking,self-splicing splicing introns are not enzymes(catalysts),because they mediate only one round of RNA processing. • The chemistry of group II intron splicing and the RNA intermediates produced are the same as that of the nuclear pre-mRNA.The introns uses an A residue within the branch site to attack the phosphodiester bond at the boundary between its 5’end 5 and the end of the 5’exon5 exon-that is,at the 5’splice 5 site.Then it forms a lariat and is followed by a second reaction in which the newly freed 3’OH 3 of the exon attacks the 3’splice 3 site,releasing the intron as a lariat and fusing the 3’ 3 and 5’ 5 exons.
Generated by Foxit PDF Creator © Foxit Software http://www.foxitsoftware.com For evaluation only. 3.3Group I introns release a linear intron rather than a lariat Instead of using a branch point A, group I introns use a free G nucleotide or nucleoside. This G species is bound by the RNA and its 3’OH group is present to the 5’ 5 splice site. The two-step transesterification reactions are the same as that of splicing of the group II intron and pre-mRNA introns.
Page 1 and 2: Generated by Foxit PDF Creator © F
Page 5 and 6: Conceptions: Generated by Foxit PDF
Page 15 and 16: 2.1 RNA splicing is carried out by
Page 25: TABLE 13-1 Generated by Foxit PDF C
Page 67: Generated by Foxit PDF Creator © F

splice site

Create successful ePaper yourself

Delete template?

Save as template?