16 TH INTERNATIONAL SYMPOSIUM ON CAROTENOIDS atomic force microscopy (AFM) enabled us to characterize adhesion interactions between unruptured vesicles and the AFM tip. Studies revealed a significant change in the dependence of adhesion force on temperature caused by the presence of β-carotene in the lipid bilayer. REFERENCES GRUSZECKI WI, STRZAŁKA K. 2005. Carotenoids as modulators of lipid membrane physical properties. Biochimica et Biophysica Acta 1740: 108-115. KOSTECKA-GUGALA A, LATOWSKI D, STRZALKA K. 2003. Thermotropic phase behaviour of α-dipalmitoylphosphatidylcholine multibilayers is influenced to various extents by carotenoids containing different structural features – evidence from differential scanning calorimetry. Biochimica et Biophysica Acta 1609: 193-202. 8.3. Spectroscopic properties of astaxanthin aggregates in hydrated solvents Milan Durchan1 , Babak Minofar2 , Tomáš Manèal3 , Lucie Tìsnohlídková4 , Tomáš Polívka4 1 Institute of Plant Molecular Biology, Biology Centre Acad. Sci. Czech Rep., Branišovská 31, 37005 Èeskì Budìjovice, Czech Republic, durchan@umbr.cas.cz 2 Department of Chemistry, Faculty of Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi, Fukuoka, Japan 812-8581, minofar@chem.kyushu-univ.jp 3 Institute of Physics, Faculty of Mathematics and Physics, Charles University, Ke Karlovu 5 121 16 Prague, Czech Republic, mancal@karlov.mff.cuni.cz 4 Institute of Physical Biology, University of South Bohemia, Zámek 136, 37333 Nové Hrady, Czech Republic, polivka@ufb.jcu.cz It is well known that carotenoids form aggregates when dissolved in hydrated polar solvents, and that aggregation is characterized by dramatic changes in their absorption spectra. In this study, aggregates of the carotenoid astaxanthin were studied. Depending on water content and organic solvent used for preparations of primary stock solution, two types of aggregates were produced: Haggregates with absorption maximum around 390 nm, and Jaggregates with red-shifted absorption band peaking at wavelengths >550 nm. The large shifts in respect of absortion maximum of monomeric astaxanthin (470-495 nm depending on solvent) are caused by excitonic interaction between aggregated molecules as demonstrated also by CD spectra. While in hydrated acetone and methanol only H-aggregates were generated, ambivalent behavior was observed in hydrated dimethyl sulfoxide (DMSO) in which both types of aggregates are observed and their ratio could be controlled by varying the water content. We applied molecular modeling simulations to elucidate structure of astax- SESSION 8 anthin dimer in water, and resulting structure was used as a basis for calculations of absorption spectra. Absorption spectra of astaxanthin aggregates in hydrated DMSO were calculated for various structures, including structural disorder, using excitonic model. The resonance interaction energy between astaxanthin monomers was determined by quantum chemical calculation, and both underdamped intramolecular vibrational modes and the overdamped solvent modes were included to fit the absorption lineshape. For selected astaxanthin aggregates, excited-state dynamics were studied by femtosecond transient absorption spectroscopy. 8.4. Violaxanthin and diadinoxanthin de-epoxidation in various model lipid systems Dariusz Latowski1 , Reimund Goss2 , Kazimierz Strzałka1 1Department of Plant Physiology and Biochemistry, Jagiellonian University, Gronostajowa 7, 30-387 Kraków, Poland, dariuszlatowski@gmail.com, kazimierzstrzalka@gmail.com 2Institute of Biology I, Plant Physiology, University of Leipzig, Johannisallee 21-23, 04103, Leipzig, Germany, rgoss@rz.uni-leipzig.de The xanthophyll cycle is an important photoprotective process functioning in plants. One of its forms, the violaxanthin (Vx) cycle, involves interconversion between: Vx, antheraxanthin (Ax) and zeaxanthin (Zx). Another kind of the xanthophyll cycle is the diadinoxanthin (Ddx) cycle in which interconversion between Ddx and diatoxanthin (Dtx) occurs. In this study an information on molecular mechanism and regulation of these two types of the xanthophyll cycle is presented. The influence of lipids on the deepoxidation of the xanthophyll cycle pigments was investigated, with special focus put on the significance of physical properties of the aggregates formed by inverted lipid micelles, which are necessary for activity of tha xanthophyll cycle enzymes. In particular, thickness of the hydrophobic fraction of the aggregates, size of the inverted micelles, suggested by mathematical description of the structures and solubility of Vx and Ddx in various kind of lipids were studied. Obtained results show that the rate of de-epoxidation is strongly dependent on the physicochemical properties of the lipids used. The key role for enzyme activation play non-bilayer lipids and the parameters of inverted micelles created by them, such as thickness, molecular dynamics of hydrophobic core and their diameter. The presented results show that MGDG and other non-lamellar lipids like different forms of phosphatidylethanolamine are necessary for the Vx and Ddx de-epoxidation because they provide the three-dimensional structures, which are needed for the binding of de-epoxidases and for the accessibility of Vx and Ddx to these enzymes. 110 <strong>ACTA</strong> <strong>BIOLOGICA</strong> <strong>CRACOVIENSIA</strong> Series Botanica
Index of Authors Abe K 44 Abramchik L 43 Abramoviè H 54 Adamkiewicz P 109 Agócs A 49, 51, 56, 57, 61 Ahmadi MR 28 Aizawa K 75, 79, 80 Al-Babili S 83, 88 Albrecht D 26 Alcalde E 87 Alder A 83 Allender C 88 Alonso-Álvarez C 51 Alos E 86 Alquezar B 86, 87 Alsvik IL 63 Amengual J 22, 77 Amotz AB 71 Andrei S 101 Arango J 86 Augustyńska D 109 Avalos J 88 Awsiuk M 18 Aydemir G 21, 28 Babino D 100 Bai C 85, 93 Bakker L 98 Baldermann S 87 Ballottari M 33 Bär C 86 Baranska M 51, 53 Baranski R 21, 53, 88 Barker M 88 Barrero AF 87 Barshack I 71 Bartók EM 28 Bashmakov D 78 Bassi R 33 Bazyar AA 28 Berendschot TTJM 97 Berera R 36 Berger RG 60 Bernardeau-Esteller J 40 Bernstein PS 11, 100, 101 Berry P 18 Betke A 34, 36 Beyer P 86 Bialek-Bylka GE 37 Biehler E 21 Blaner WS 17, 20 Blomhoff R 21 Boddy A 18 Böhm F 77 Böhm V 22, 70, 79, 107 Bohn T 21, 22 Bojko M 91 Bone R 97, 100 Bonet ML 18, 22 Borel P 19, 24 Bösch-Saadatmandi C 22 Boulton ME 98 Bowman M 41, 85 Bragagnolo N 53, 54 Bremer P 88 Bresgen N 76 Bronson RT 72 Brulc L 54 Bunea A 101 Burda K 38, 109 Burt A 22 Butler MW 23 Cacciola F 56 Cagir A 55 Campillo JA 40 Canela R 85 Capell T 85, 93 Carail M 75 Caris-Veyrat C 23, 24, 28, 75 Carle R 49 Carlsen H 21 Carmona L 86 Carne A 88, 91 Carne A 91 Cazzonelli CI 83 Cerdá-Olmedo E 87 Èerneliè K 62 Chacon B 54 Chan GM 100 Chandra A 25 Chen C 25 Chen C-H 77 Chen THH 90 Chisté RC 59 Choi E 52 Christou P 85, 93 Chung J 29 Collins AR 17 Cordell H 22 Corrochano LM 91 Craft NE 54 Crawford K 55 Cress E 20 Crifo C 76 Curley Jr RW 19, 20 Czech U 28 Dalay MC 55 Dalke I 35 Dall'Osto L 33 Daly A 22 D'Ambrosio DN 20 Dangles O 24, 75 Danilenko M 68 Dawadi S 12 Day C 22 De Spirt S 24, 71 Degrou A 23 dela Sena C 19 Deli J 27, 41, 49, 51, 55, 56, 57, 58, 60, 61, 71 Dembinska-Kiec A 18, 28 Deshpande J 24 Díaz-Sánchez V 88 Dobrucki JW 45 Drahos L 56 Duda M 106 Dugo G 56 Dulak J 67 Dulińska-Litewka J 67 Durchan M 110 Dymova O 35, 37 Dymova OV 26, 37 Dynarowicz-Łątka P 59 Eckl P 76 Edge R 77 Ehlers F 36, 42 Eijssen L 22 Elgsaeter A 52 Eliassen AH 25 Enriquez MM 33 Erdman Jr JW 11 Erdogan A 55 Ernst H 29, 49, 63, 72 Eroglu A 19 Eroglu AE 55 Estrada AF 88 Evelo C 22 Farre G 93 Fechner M 70, 107 Fernández-Orozco R 89 Fernández-Palacios H 78 Fiedor J 38 Fiedor L 38 Fleischmann P 87 Fleshman MK 19, 20 Flinterman M 69 Focsan L 41 Frank HA 33, 38, 40 Frederick JM 101 Fröhlich K 70, 79, 107 Fuciman M 33 Fujii R 38, 108 Fulton AB 100 Furubayashi M 93 Gall A 38, 44 Gallardo-Guerrero L 89 Garaffo M 56 Garama D 88, 91 García-de Blas E 51 Gardiner R 39 Gellenbeck K 25 Gellermann W 98 Georgé S 23 Gibert J 97 Giuffrida D 56 Giuliano G 83 Gohto Y 98 Golibrzuch K 42 Golovko T 35, 37 Gombos Z 33, 90 Goncalves A 19 Gonchar MV 89 González-Miret ML 59 Goralska J 18, 28 Gospodarek M 45 Goss R 34, 43, 110 Goupy P 75 Gouranton E 28 Graham DL 79 Green RC 20 Gruca A 28 Grudzinski W 45 Gruszecki WI 30, 45, 106, 108, 109 Gryczynski I 45 Gryczynski Z 45 Grzebelus E 53 Gulyás-Fekete G 56, 57, 60 Gurak PD 59 Gural S 89 Gwizdala M 35, 36 Gyémánt N 27 Háda M 51, 57 Hammond Jr BR 97 Han RM 77 Harari A 71 Harats D 71 Harrison EH 19, 20 Hashimoto H 38, 44, 108 Hausman D 20 Hazra A 25 Heinrich U 24 Helyes Z 58 Hendrickson S 25 Heredia FJ 58, 59 Herrador MM 87 Hesketh J 18, 22 Hessel S 22 Hirschberg J 11, 90 Hoffmann L 21, 22 Holzwarth AR 33 Hong I 52 Hongo N 30 Horibe T 108 Hornero-Méndez D 89 Horváth G 27, 57, 58, 60 Hosokawa M 70, 75 Hruszkewycz DP 19 Huner NPA 39 Hunter CN 43, 108 Hurry V 39 Iha M 38 Ilioaia C 38 Illyés TZ 56 Inakuma T 79, 80 Intra J 25 Iskandar A 70, 72 Ivanov AG 39 Iwasaki Y 80 Jahns P 108 Jedynak P 39 Jeknić Z 90 Jemioła-Rzemińska M 34, 41, 43, 88, 109 Jevremović S 90 Johnson EJ 17, 20, 29, 100 Johnson MA 20 Józkowicz A 67 Jubran H 90 Jung SY 52 Kabashnikova L 43 Kaczor A 51
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Patronage Mayor of the City of Krak
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Organizing Committee Małgorzata Ba
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Session 1 Nutritional Carotenoids a
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