16 TH INTERNATIONAL SYMPOSIUM ON CAROTENOIDS RAO AV, RAY MR, RAO LG. Lycopene. 2006. Advances in Food and Nutrition Research 51: 99-164. KOPEC RE, RIEDL KM et al. 2010. Identification and quantification of apo-lycopenals in fruits, vegetables, and human plasma. Journal of Agricultural and Food Chemistry 58: 3290-3296. 5.6. Development of singlet oxygen absorption capacity (SOAC) assay method and the quenching activity of singlet oxygen by carotenoids and vegetables Shingo Takahashi1 , Yuko Iwasaki1 , Aya Ouchi2 , Koichi Aizawa 1 , Takahiro Inakuma1 , Shin-ichi Nagaoka2 , Junji Terao 3 , Kazuo Mukai2 1 Research Institute, Kagome Co., Ltd., 17 Nishitomiyama, Nasushiobara, Tochigi, 329-2762, Japan, Shingo_Takahashi@kagome.co.jp 2 Department of Chemistry, Faculty of Science, Ehime University, 2-5 Bunkyo-cho, Matsuyama, Ehime, 790-8577, Japan, mukai-k@dpc.ehime-u.ac.jp 3 Department of Food Science, Graduate School of Nutrition and Bioscience, The University of Tokushima, Tokushima, 770-8503, Japan Lipid peroxyl radical (LOO • ) and singlet oxygen ( 1 O 2 ) are two well-known representative reactive oxygen species (ROS) generated in biological systems. In recent years, the method to assess the total oxygen radical absorption capacity (ORAC) of foods and SESSION 5 plants has been developed. On the other hand, the method to assess the 1 O 2 -quenching activity of foods and plants which include carotenoids and phenolic antioxidants has not been developed. In the present work, measurements of the quenching rate of 1 O 2 (k Q ) for eight carotenoids were performed by using the competition reaction method. The k Q values of carotenoids obtained were 50-100 times greater than that of α-tocopherol which was used as a standard compound. Furthermore, we measured the k Q values of three kinds of carotenoid-rich vegetable extracts and the concentrations of seven kinds of carotenoids included in vegetable extracts, using a HPLC technique. From the results, it has been clarified that the total 1 O2 -quenching activity for vegetable extracts may be explained as Car-i the sum of the product {∑ kQ [Car-i]i } of the rate constant Car-i (kQ ) and the concentration ([Car (i)]) of carotenoids included in vegetable extracts. Based on the results, a new assay method that can quantify the singlet oxygen absorption capacity (SOAC) of foods and plants including carotenoids and phenolic antioxidants was proposed. REFERENCES OUCHI A, AIZAWA K, IWASAKI Y, INAKUMA T, TERAO J, NAGAOKA S, MUKAI K. 2010. Kinetic study of the quenching reaction of singlet oxygen by carotenoids and food extracts in solution. Development of a singlet oxygen absorption capacity (SOAC) assay method. J. Agric. Food Chem. 58: 9967-9978. AIZAWA K, IWASAKI Y, OUCHI A, INAKUMA T, NAGAOKA S, TERAO J, MUKAI K. 2011. Development of singlet oxygen absorption capacity (SOAC) assay method. 2. Measurements of the SOAC values for carotenoids and food extracts. J. Agric. Food Chem. 59: 3717- 3729. 80 <strong>ACTA</strong> <strong>BIOLOGICA</strong> <strong>CRACOVIENSIA</strong> Series Botanica
Session 6 Biosynthesis, Genetics, and Metabolism of Carotenoids
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Patronage Mayor of the City of Krak
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Organizing Committee Małgorzata Ba
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Plenary lectures
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