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16 TH INTERNATIONAL SYMPOSIUM ON CAROTENOIDS pogenesis enzyme, was significantly decreased in ALA supplemented group, as compared to controls. Since Sirt1 plays a key role in lipid homeostasis, the present study suggests that ALA protects against the development of steatosis in ob/ob mice by upregulating Sirt1 gene expression. 1.25. Fluorescence spectroscopy of polyene antibiotic drug amphotericin B Piotr Waśko, Wiesław I. Gruszecki Department of Biophysics, Institute of Physics, Maria Curie – Skłodowska University in Lublin, Poland, piotr.wasko@gmail.com, wieslaw.gruszecki@umcs.pl Amphotericin B (AmB) is an antifungal antibiotic commonly used in treating deep-seated mycosis. It has many side effects such as breath and heart rate disorder, it is nephrotoxic and hepatotoxic. That is why, it is important to discover mechanisms of its interaction with fungal and human cells. From other investigations, it is known that AmB binds to lipid membranes. The structure of energetic states of the drug in diffrent molecular forms and model of its interaction with lipid membranes are still under detailed investigations. Auto-fluorescence of AmB may be also used to investigate the structure of energetic states of the drug in diffrent molecular forms. Auto-fluorescence of AmB incorporated to the DPPC liposomes may be used to investigate dynamic and structural properties of lipid membranes and simultaneously molecular organization of the drug with using fluorescence anisotropy. The samples we examinated included AmB in pH7 , pH12 water buffers and DPPC liposomes with AmB in concentration 5mol% with respect to lipid. The conclusions of our investigations are: 1. Fluorescence anisotropy spectra of sample in pH12 buffer show the presence of two molecular forms of the durg, though solution with such high pH level should containe only monomeric form. It also shows that 521 nm peak in fluorescence emission spectra is not a part of emission band of the monomeric form, 2. Comparison of the fluorescence exitation ane 1-T spectra confirms that the sample in pH12 buffer containes both the monomeric and the dimeric forms of AmB, 3. Fluorescence anisotropy spectra of AmB incorporated to DPPC liposomes suggests that dimeric form of the drug binds stronger to the membranes as compared to the monomeric form. 1.26. Lutein supplementation fosters neuroprotection and improves associative and spatial learning and memory performance in aged mice Adrian Wyss, Annis O. Mechan, Nicole Seifert, Henry Rieger, Jenny Piussi, M. Hasan Mohajeri DSM Nutritional Products, NIC R&D Human Nutrition and Health, P.O. Box 2676, CH-4002 Basel, Switzerland, adrian.wyss@dsm.com, hasan.mohajeri@dsm.com Introduction: Due to changes in demographic structure, the decline of cognitive abilities with age is among the largest socio-economic problems of modern societies. Therefore, it is desirable to search for safe nutritional compounds to delay or prevent age-associated reductions in mental performance. Beneficial effects of lutein, a hydroxylated Carotenoid (Xanthophyll) which is present in several food sources including broccoli, spinach, kale and pepper, on brain functions have been previously studied in several paradigms including animal models of neurodegenerative disorders. Methods: FloraGlo® Lutein was tested in a glutamate intoxication assay there primary cortical neurons were pretreated with Lutein. We have further tested aged animals, after chronic dietary supplementation with 9 mg/kg b.w /d. FloraGlo® Lutein in the IntelliCage System. After a 2 weeks adaptation period animals had to perform for at least 4 weeks, in four different behavioral paradigms examining exploratory-, associative-, stress-relatedand spatial- learning and memory. Results: FloraGlo® Lutein supports neuronal survival through protection against oxidative stress and against glutamate intoxication. Moreover, FloraGlo® Lutein significantly promotes associative learning and memory, learning and memory under stress and spatial- learning and memory but is ineffective in supporting other learning paradigms tested in the IntelliCage System. Conclusion: These data suggest that supplementation with FloraGlo® Lutein may be helpful in improving specific aspects of learning and memory in daily life situations and further may help to slow the normally occurring age-associated deterioration of cognitive performance. 1.27. Cosmetic benefits of astaxanthin oral supplementation on humans subjects Kumi Tominaga, Nobuko Hongo, Mariko Karato, Eiji Yamashita Fuji Chemical Industry Co. Ltd., 55 Yokohouonji, Kamiichi, Toyama 930-0397, Japane, yamashita@fujichemical.co.jp A randomized double-blind placebo controlled study involving 36 healthy male subjects for 6 weeks was performed. Crow's feet wrinkle and elasticity; and transepidermal water loss (TEWL) improved after 6 mg of astaxanthin daily supplementation. Moisture content and sebum oil level at the cheek zone showed strong tendencies for improvement. These results suggest that astaxanthin derived from Haematococcuspluvialis may improve the skin condition in not only in women but also in men. 1.28. Cosmetic benefits of oral supplementation combined with topical treatment of astaxanthin on humans subjects Kumi Tominaga, Nobuko Hongo, Mariko Karato, Eiji Yamashita Fuji Chemical Industry Co. Ltd., 55 Yokohouonji, Kamiichi, Toyama 930-0397, Japane, yamashita@fujichemical.co.jp SESSION 1 An open-label non-controlled study involving 30 healthy female subjects for 8 weeks was performed. Significant improvements were observed by combining 6 mg per day oral supplementation and 2 ml (350 μM solution) per day topical application of astaxanthin. Astaxanthin derived from the microalgae, Haematococcus pluvialis showed improvement in skin wrinkle reduction (crow's feet at week 8), age spot size (cheek at week-8), and improved elasticity (crow's feet at week-8), skin texture (cheek at week-4), moisture content of corneocyte layer (cheek in 10 dry skin subjects at week-8) and corneocyte condition (cheek at week-8). These results suggest that astaxanthin derived from H. pluvialis can improve skin condition in all layers such as corneocyte layer, epidermis, basal layer and dermis by combining oral supplementation and topical treatment. 30 ACTA BIOLOGICA CRACOVIENSIA Series Botanica
Session 2 Photosynthesis, Photochemistry, and Photoprotection by Carotenoids Professor Trevor W. Goodwin in Memoriam
Page 1 and 2: ACTA BIOLOGICA CRACOVIENSIA SERIES
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Page 5 and 6: Patronage Mayor of the City of Krak
Page 7 and 8: Organizing Committee Małgorzata Ba
Page 9: Plenary lectures
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Page 15 and 16: Session 1 Nutritional Carotenoids a
Page 17 and 18: NUTRITIONAL CAROTENOIDS AND THEIR I
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Page 48 and 49: Professor Dr. Hans-Dieter Martin 19
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16 TH INTERNATIONAL SYMPOSIUM ON CA
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BIOSYNTHESIS, GENETICS, AND METABOL
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Session 7 Carotenoids and Vision
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MODEL SYSTEMS, COMPUTATIONAL AND IN
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Index of Authors Abe K 44 Abramchik
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Szõke E 58 Szolcsány J 58 Szurkow
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ROMEO JT. 1973. A chemotaxonomic st
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