ACTA BIOLOGICA CRACOVIENSIA

16 TH INTERNATIONAL SYMPOSIUM ON CAROTENOIDS
pogenesis enzyme, was significantly decreased in ALA supplemented
group, as compared to controls. Since Sirt1 plays a key
role in lipid homeostasis, the present study suggests that ALA
protects against the development of steatosis in ob/ob mice by upregulating
Sirt1 gene expression.
1.25.
Fluorescence spectroscopy of polyene
antibiotic drug amphotericin B
Piotr Waśko, Wiesław I. Gruszecki
Department of Biophysics, Institute of Physics, Maria Curie –
Skłodowska University in Lublin, Poland, piotr.wasko@gmail.com,
wieslaw.gruszecki@umcs.pl
Amphotericin B (AmB) is an antifungal antibiotic commonly used in
treating deep-seated mycosis. It has many side effects such as breath
and heart rate disorder, it is nephrotoxic and hepatotoxic. That is
why, it is important to discover mechanisms of its interaction with
fungal and human cells. From other investigations, it is known that
AmB binds to lipid membranes. The structure of energetic states of
the drug in diffrent molecular forms and model of its interaction with
lipid membranes are still under detailed investigations.
Auto-fluorescence of AmB may be also used to investigate the
structure of energetic states of the drug in diffrent molecular
forms. Auto-fluorescence of AmB incorporated to the DPPC liposomes
may be used to investigate dynamic and structural properties
of lipid membranes and simultaneously molecular organization
of the drug with using fluorescence anisotropy.
The samples we examinated included AmB in pH7 , pH12
water buffers and DPPC liposomes with AmB in concentration
5mol% with respect to lipid.
The conclusions of our investigations are:
1. Fluorescence anisotropy spectra of sample in pH12 buffer
show the presence of two molecular forms of the durg, though
solution with such high pH level should containe only
monomeric form. It also shows that 521 nm peak in fluorescence
emission spectra is not a part of emission band of the
monomeric form,
2. Comparison of the fluorescence exitation ane 1-T spectra confirms
that the sample in pH12 buffer containes both the
monomeric and the dimeric forms of AmB,
3. Fluorescence anisotropy spectra of AmB incorporated to
DPPC liposomes suggests that dimeric form of the drug binds
stronger to the membranes as compared to the monomeric
form.
1.26.
Lutein supplementation fosters neuroprotection
and improves associative and spatial learning
and memory performance in aged mice
Adrian Wyss, Annis O. Mechan, Nicole Seifert, Henry Rieger,
Jenny Piussi, M. Hasan Mohajeri
DSM Nutritional Products, NIC R&D Human Nutrition and Health,
P.O. Box 2676, CH-4002 Basel, Switzerland,
adrian.wyss@dsm.com, hasan.mohajeri@dsm.com
Introduction: Due to changes in demographic structure, the decline
of cognitive abilities with age is among the largest socio-economic
problems of modern societies. Therefore, it is desirable to search for
safe nutritional compounds to delay or prevent age-associated
reductions in mental performance. Beneficial effects of lutein, a
hydroxylated Carotenoid (Xanthophyll) which is present in several
food sources including broccoli, spinach, kale and pepper, on brain
functions have been previously studied in several paradigms including
animal models of neurodegenerative disorders.
Methods: FloraGlo® Lutein was tested in a glutamate intoxication
assay there primary cortical neurons were pretreated with
Lutein. We have further tested aged animals, after chronic dietary
supplementation with 9 mg/kg b.w /d. FloraGlo® Lutein in the
IntelliCage System. After a 2 weeks adaptation period animals
had to perform for at least 4 weeks, in four different behavioral
paradigms examining exploratory-, associative-, stress-relatedand
spatial- learning and memory.
Results: FloraGlo® Lutein supports neuronal survival
through protection against oxidative stress and against glutamate
intoxication. Moreover, FloraGlo® Lutein significantly promotes
associative learning and memory, learning and memory under
stress and spatial- learning and memory but is ineffective in supporting
other learning paradigms tested in the IntelliCage System.
Conclusion: These data suggest that supplementation with
FloraGlo® Lutein may be helpful in improving specific aspects of
learning and memory in daily life situations and further may help
to slow the normally occurring age-associated deterioration of
cognitive performance.
1.27.
Cosmetic benefits of astaxanthin oral
supplementation on humans subjects
Kumi Tominaga, Nobuko Hongo, Mariko Karato,
Eiji Yamashita
Fuji Chemical Industry Co. Ltd., 55 Yokohouonji, Kamiichi,
Toyama 930-0397, Japane, yamashita@fujichemical.co.jp
A randomized double-blind placebo controlled study involving 36
healthy male subjects for 6 weeks was performed. Crow's feet
wrinkle and elasticity; and transepidermal water loss (TEWL)
improved after 6 mg of astaxanthin daily supplementation.
Moisture content and sebum oil level at the cheek zone showed
strong tendencies for improvement. These results suggest that
astaxanthin derived from Haematococcuspluvialis may improve
the skin condition in not only in women but also in men.
1.28.
Cosmetic benefits of oral supplementation
combined with topical treatment of
astaxanthin on humans subjects
Kumi Tominaga, Nobuko Hongo, Mariko Karato,
Eiji Yamashita
Fuji Chemical Industry Co. Ltd., 55 Yokohouonji, Kamiichi,
Toyama 930-0397, Japane, yamashita@fujichemical.co.jp
SESSION 1
An open-label non-controlled study involving 30 healthy female subjects
for 8 weeks was performed. Significant improvements were
observed by combining 6 mg per day oral supplementation and 2 ml
(350 μM solution) per day topical application of astaxanthin.
Astaxanthin derived from the microalgae, Haematococcus pluvialis
showed improvement in skin wrinkle reduction (crow's feet at week
8), age spot size (cheek at week-8), and improved elasticity (crow's
feet at week-8), skin texture (cheek at week-4), moisture content of
corneocyte layer (cheek in 10 dry skin subjects at week-8) and corneocyte
condition (cheek at week-8). These results suggest that
astaxanthin derived from H. pluvialis can improve skin condition in
all layers such as corneocyte layer, epidermis, basal layer and dermis
by combining oral supplementation and topical treatment.
30 ACTA BIOLOGICA CRACOVIENSIA Series Botanica