ACTA BIOLOGICA CRACOVIENSIA

CHEMISTRY, ANALYSIS, CHEMICAL SYNTHESIS, AND INDUSTRIAL PRODUCTION OF CAROTENOIDS
These data allow us to suggest that B corresponds to 3-hydroxyκ,κ-caroten-6,6'-dione
and C is κ,κ-caroten-6,6'-dione. The
carotenoids B and C were also characterized by their 1H NMR and
CD spectra. The presence of the direct metabolic precursor of B
and C in red mamey supports this proposal, as well.
This study, on the part of the Hungarian authors was supported by the
grant OTKA K 83898 (Hungarian Scientific Research Foundation). We
thank Dr. L. Drahos for performing the HRESITOFMS measurements.
3.22.
New sources of κ-ring carotenoids in Panama's
biodiversity
Enrique Murillo, Moisés Watts, Verónica Mosquera,
José Robinson, Reinaldo McLean
Faculty of Natural Sciences, Exacts and Technology, University
of Panama, Panama City Panama, emurillo29@hotmail.com,
moybuzz@hotmai.com, luchochem@hotmail.com
There are few sources of carotenoids with κ-ring end groups.
Capsanthin, capsorubin, capsanthin 5,6 epoxide and cryptocapsin
are the most known carotenoids with κ-ring end groups. The presence
of a carbonyl group conjugated with the polyenes chain, is
responsible of the red color of these carotenoids and vegetables
that contain them. Panama is a humid tropical country with a large
native biodiversity, where there are many plants with red parts
(fruit, leaves, flowers and seeds), which have not been investigated.
Our group has studied the carotenoids of several of these sources,
had been isolated and identified the main carotenoids ?, combining
open column chromatography, TLC, HPLC-DAD, HPLC-MS and
qualitative tests, comparing with standards. We found that redbrownish
leaves of Zamia (skinneri, dressleri and neurophyllidia),
plants considered living fossils, containing capsorubin, capsanthin
and capsanthin 5,6-epoxy, where the main carotenoid is capsorubin.
This is the first report on the presence of carotenoids with κring
in leaves. The Chinese passion fruit (Cionosicyos machranthus)
contains cryptocapsin, capsanthin and cryptocapsin 5,6epoxy.
The red mamey fruit (Pouteria sapota), appreciated for its
pleasant taste, contains cryptocapsin, sapotexanthin, cryptoxanthin
5,6-epoxy, capsanthin 5,6 epoxy and others. This is the first
report of edible fruits, with high content in cryptocapsin. This
carotenoid has been reported in the paprika, but in trace amounts.
The inflorescence of Jipijapa (Carludovica palmata) contains capsorubin,
capsanthin and capsanthin 5,6-epoxy. The seeds of
Zamias (skinneri, neurophyllidia, nesófila, acuminata,
fairchildiana and oblikua) contain capsorubin, capsanthin and
capsanthin 5,6-epoxy. Probably the striking color of these parts of
the plant, helps its spread by animals. The fruit of niguito (Cordia
collococca) contains capsorubin and capsanthin. This is a wild
fruit not consumed by humans very often, but greatly appreciated
by birds. The results show that the κ-ring carotenoids are found in
all of the plant part (fruit, leaf, seeds and flowers).
3.23.
Carotenoid-cysteine conjugates
Afshin Zand, Attila Agócs, József Deli, Veronika Nagy
Department of Biochemistry and Medical Chemistry, Medical
School, University of Pécs, Szigeti út 12, H-7624 Pécs, Hungary,
af.zand@gmail.com, vera.nagy@aok.pte.hu,
jozsef.deli@aok.pte.hu
The hydrophobic natural carotenoids are efficient antioxidants,
however, chemical synthesis of their water-soluble derivatives is
Vol. 53, suppl. 1, 2011
reasonable to gain better bioavailability. Isozeaxanthin gives
allylic cation on acidic treatment which reacts readily with thiol
nucleophiles (Nagy et al., 2010). Using N-acetylcysteine as nucleophile
the obtained products are carotenoid-cysteine conjugates
in which the amin oacid moiety connects to the carotenoid
through sulphur in position 4. The water solublity of the product
can be increased by deprotection of the amino group.
The antioxidant activity of the products were examined on human
liver cells in hydrogenperoxide induced oxidative stress. The
intake of the synthesized products by the cells was facilitated by
preparing lyposomes (C. Socaciu et al., 1999).
We thank Mrs. Izabella Solti for her help in biological essays. This
study was supported by OTKA K 83898 (Hungarian National Research
Foundation).
REFERENCES
NAGY V, AGÓCS A, TURCSI E, DELI J. 2010. Experiments on the synthesis
of carotenoid glycosides. Tetrahedron Letters 51: 2020-2022.
SOCACIU C, LAUSCH C, DIEHL HA. 1999. Carotenoids in DPPC vesicles:
membrane dynamics. Spectrochimica Acta Part A 55: 2289-2297.
3.24.
July 17– 22, 2011, Krakow, Poland
Spectroscopy analysis for simultaneous
determination of lycopene and β-Carotene
in fungal biomass of Blakeslea trispora
Iaroslav Soroka, Valeriy Narushin, Yuriy Turiyansky,
Alexey Tyurenkov
Vitan Group, Vita-Market Ltd., Yuzhnoe Shosse 1, Zaporozhye,
69032 Ukraine, narushin@vitan.ru
Blakeslea trispora is a good source for producing lycopene and
β-carotene. The objective of this research was to elaborate
a method for the simultaneous determination of lycopene and
β-carotene using UV-vis spectrophotometer.
The standard solutions of the mixture of different concentrations
of β-carotene and lycopene were measured with the UV-vis
method and a correlation formula for the extinction coefficients of
1% standard solution of lycopene in the solvent (hexane) and the
ratios of the optical densities at the character peaks of 470 and
502 nm was defined:
in which A1% lyc is the extinction coefficient of a 1% lycopene solution;
D470 is the optical density of the spectrum at λ=470 nm;
D502 is the optical density of the spectrum at λ=502 nm.
Substituting the result into the classic formula, gives a possibility
to calculate the concentrations of lycopene in the mixture:
in which Clyc is the percentage of lycopene in %; D470 is the optical
density of the investigated solution at the wavelength of 470
nm; V is the solvent (hexane) volume, being spent for the preparation
of the investigated solution in ml; m is the weight of the
sample in g; l is the thickness of the cuvette for the optical density
measurements in cm; A 1% lyc-tab is the table extinction coefficient
of the 1% solution of pure lycopene, equals to 3450.
Then the optical density for β-carotene (Dβ-car ) is calculated as:
D β-car = D 470 – D lyc
in which D β-car is the optical density for β-carotene at the wavelength
of 470 nm; D 470 is the optical density of the mixed
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