ACTA BIOLOGICA CRACOVIENSIA

16 TH INTERNATIONAL SYMPOSIUM ON CAROTENOIDS
1.19.
Health promotion by lycopene
Hoyoku Nishino
Ritsumeikan University & Kyoto Prefectural University of
Medicine, Nojihigashi 1-1-1, Kusatsu, Shiga 525-8577, Japan,
cha-nishino@nifty.com
Lycopene has been widely investigated as a possible cancer preventive
agent, especially in prostate cancer prevention. We have
already reported that lycopene is also effective for prevention
against hepatitis C virus-induced liver cancer (Nishino et al.,
2009). Recently, lycopene has been shown to be an useful agent
for various lifestyle- related diseases, such as cardiovascular diseases,
as well as cancer. We have also found that lycopene is
promising as a preventive agent against osteoporosis. Therefore,
lycopene seems to be an important carotenoid in preventive medicine.
Health promotion by lycopene will be discussed in this
presentation.
REFERENCES
NISHINO H, MURAKOSHI M, TOKUDA H, SATOMI Y. 2009. Cancer prevention
by carotenoids. Arch. Biochem. Biophys. 483: 165-168.
1.20.
Dihydro-lycopenoids are lycopene-metabolites
Gamze Aydemir1 , Emöke Márta Bartók1 , Erik Reynaud2 ,
Erwan Gouranton 3 , Catherine Caris-Veyrat2 ,
Jean-Francois Landrier 3 , Ralph Rühl1,4 1Laboratory of Nutritional Bioactivation and Bioanalysis,
Department of Biochemistry and Molecular Biology, Medical and
Health Science Center, University of Debrecen, Nagyerdei Krt,
4032 Debrecen, Hungary, rruehl@dote.hu, gaydinn@hotmail.com
2INRA, UMR 408 safety and quality of plant products, 84000
Avignon, France
3INRA, UMR1260 "Nutriment lipidiques et prevention des maladies
metaboliques", 13385 Marseille, France
4Apoptosis and Genomics Research Group of the Hungarian
Academy of Science, Debrecen, Hungary
Lycopene has been shown to be a potent activator of RAREmediated
signaling in RARE-LUC reporter mice and therefore
we are in search for lycopene metabolic pathways and bio-active
lycopene-metabolites. Our initial targets were the fully conjugated
and previously detected apo-lycopenoids. Various apolycopenoids
were synthesized for this study ranging from apo-
10´-, apo-12´- and apo-14´-lycopenoids. In these experiments
lycopene was supplemented either orally or intra-venously for
better absorption to mice and administered to cultured human
adipocytes. In various organs of the lycopene-supplemented
mice we could not detect any apo-lycopenoic acids using highly
sensitive and specific HPLC MS-MS techniques. Fortunately, in
the white adipose tissue of lycopene-supplemented animals we
could identify a large peak using single ion monitoring which
has a molecular weight of 2 Da higher then apo-10´-lycopenoic
acid. Based on our HPLC-MS data and additional UV detection
we conclude that this peak might be 7,8-dihydro-apo-10´lycopenoic
acid. We suggest that dihydro-apo-10´-lycopenoic
acids might be novel lycopene-metabolites resulting in bioactive
RAR and RXR activating apo-lycopenoids, which might explain
potent RARE-activation induced by tomato preparations and
lycopene.
1.21.
Effects of carotenoids on growth performance,
mortality and carotenoid pigmentation of
rainbow trout (Onchorynchus mykiss)
Shahabeddin Safi1 , Mohammad Reza Ahmadi2 ,
Amir Abbas Bazyar3 1Department of Clinical Pathology, Faculty of Specialized
Veterinary Sciences, Science and Research Branch, Islamic Azad
University, Tehran, 1477893855, Tehran, Iran, s.safi@srbiau.ac.ir
2Department of Health and Aquatic Diseases, Faculty of
Veterinary Medicine, Tehran University, 14155-6453, Tehran, Iran,
mahmadi@ut.ac.ir
3Department of Fisheries and Environmental Sciences, Faculty of
Natural Resources, Tehran University, 31585-4314, Karaj, Iran,
bazyar@ut.ac.ir
Carotenoids are natural liposoluble pigments ranging from colorless
and yellow to red and blue, and have several biological functions
in plants and animals. Fish and other animals are unable to
biosynthesize carotenoids de novo and must obtain them from their
diet (Davies, 1985). Asataxanthin and canthaxanthin (β, β-carotene-
4,4’-dione) are the most commonly used carotenoids for pigmentation
of farmed salmonid fishes. In the present study the effects of
dietary astaxanthin supplementation on growth performance, mortality,
carotenoid pigmentation was investigated in triplicate groups
each with 1000 swim up larvae of rainbow trout , derived from five
groups of female broodstock fed diets with 0.07, 12.5, 33.3, 65.1
and 92.9 mg astaxanthin kg -1 , respectively. The first feeding fry
were fed a diet not supplemented with carotenoids. Fry were initially
sampled for astaxanthin content and initial weight, and in subsequent
15-day intervals to determine weights, condition factors (CF),
specific growth rates (SGR) and thermal growth coefficients (TGC).
Total carotenoid concentration of the larvae was highly linearly correlated
to that of eggs (r 2 =0.97, P=0.002). About 59-67% of fry
carotenoids consisted of esterified astaxanthin, and on average
39.7% of the egg carotenoids were recovered in the fry. Overall (0-
45 days) SGRs and TGCs were significantly higher (P < 0.05) in the
offspring of the four groups of females fed supplemented diets (12.5-
92.9 mg astaxanthin kg-1) than in offspring of the females fed the
non-supplemented diet. TGCs (0-45 days) within groups derived
from broodstock supplemented with astaxanthin were similar (P >
0.05), but higher than in the group derived from females fed the diet
not supplemented with astaxanthin (P < 0.05). Mortality (average
0.76%) was not significantly affected by treatment. The study indicates
that dietary supplement of astaxanthin (> 12.5 mg kg -1 ) to
maternal broodstock diets improves offspring SGR and TGC with
up to 33 and 38%, respectively.
REFERENCES
DAVIES BH. 1985. Pure Appl. Chem. 57: 679-684.
1.22.
SESSION 1
Antioxidants quercetin and beta carotene can
modulate mitochondrial function of human
preadipocytes
Agnieszka Sliwa, Joanna Goralska , Urszula Czech,
Anna Gruca, Anna Knapp, Aldona Dembinska-Kiec
Department of Clinical Biochemistry, The Jagiellonian University,
15a Kopernika Street, 31-501 Cracow, Poland
One by the reason of complication of obesity, like diabetes type 2,
could be mitochondrial dysfunction, increase ROS generation
and decrease antioxidant activity. Quercetin and beta carotene
28 ACTA BIOLOGICA CRACOVIENSIA Series Botanica