Accucore HPLC Columns - Cromlab

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More Sensitive than 5 μm or 3 μm According to the formula shown below, the sharper, taller peaks obtained with Accucore HPLC columns result in a higher signal to noise ratio (S/N) and therefore better sensitivity. c max Concentration at peak apex N Efficiency V i Injection volume L Column length d c Column internal diameter k' Capacity factor V i N c max ∝ 2 L d c (1 + k' ) mAU 399 368 169 fully porous 5 µm 100 x 2.1 mm fully porous 3 µm 100 x 2.1 mm Accucore RP-MS 2.6 µm 100 x 2.1 mm 0 1 2 3 4 5 6 7 8 Minutes Mobile phase A: Mobile phase B: water acetonitrile Gradient: Accucore RP-MS 2.6 µm 100 x 2.1 mm = 35–60 % B in 3.5 minutes fully porous 3 µm 100 x 2.1 mm = 35–60 % B in 4.0 minutes fully porous 5 µm 100 x 2.1 mm = 35–60 % B in 6.7 minutes Flow: Accucore RP-MS 2.6 µm 100 x 2.1 mm = 400 µL/min fully porous 3 µm 100 x 2.1 mm = 350 µL/min fully porous 5 µm 100 x 2.1 mm = 210 µL/min Temperature: 30 °C Injection: 1 µL Detection: Analytes: UV at 247 nm (0.1s rise time, 20 Hz) 1. Tebuthiuron 2. Metoxuron 3. Monuron 4. Chlorotoluron 5. Diuron 6. Linuron Sensitivity Column S/N (6-sigma) for Monuron Increase in Sensitivity Accucore 2.6 µm, 100 x 2.1 mm 399 136 % fully porous 3 µm, 100 x 2.1 mm 368 117 % fully porous 5 µm, 100 x 2.1 mm 169 – Better sensitivity allows reliable detection and determination of small peaks, for example low level impurities. 8
Equivalent Performance to Sub-2 μm with Lower Pressure With solid core design, tight particle size distribution and uniform packed bed Accucore HPLC columns have broadly equivalent performance to sub-2 μm columns and yet generate only a fraction of the backpressure. mAU fully porous < 2 μm 100 x 2.1 mm Accucore RP-MS 2.6 µm 100 x 2.1 mm 0 0.5 1.0 1.5 2.0 2.5 Minutes Mobile phase A: water Mobile phase B: acetonitrile Gradient: Flow: Temperature: 40 °C Injection: 1 µL Detection: Analytes: 65–95 % B in 1.7 minutes 95 % B for 0.3 minutes 500 µL/min UV at 247 nm (0.1s rise time, 20 Hz) 1. Acetophenone 2. Propiophenone 3. Butyrophenone 4. Valerophenone 5. Hexanophenone 6. Heptanophenone 7. Octanophenone Pressure Accucore RP-MS 2.6 µm, 100 x 2.1 mm Fully Porous < 2 µm, 100 x 2.1 mm Resolution (critical pair) 3.72 4.20 Run time (min) 3.50 3.50 Maximum pressure (bar) 171 338 Lower backpressure eliminates the requirement for UHPLC systems with maximum pressure ratings >600 bar. If a UHPLC system is used then the lower backpressure reduces wear on the instrument. 9
Page 1 and 2: Thermo Scientific Accucore HPLC Col
Page 3 and 4: Table of Contents Particle Evolutio
Page 5 and 6: Why Core Enhanced Technology Works
Page 7 and 8: Faster than 5 μm and 3 μm Using A
Page 9: Higher Peak Capacity than 5 μm or
Page 13 and 14: Simple Method Transfer Fast HPLC is
Page 15 and 16: Instrument Optimization Accucore HP
Page 17 and 18: Long Lasting Columns Chromatographe
Page 19 and 20: Phase Characterization Accucore pha
Page 21 and 22: HR/10 AI HS IEX (2.7) C BA IEX (7.6
Page 23 and 24: Accucore C18 Hydrophobicity HR/10 L
Page 25 and 26: Accucore aQ Hydrophobicity HR/10 Lo
Page 27 and 28: Accucore Phenyl-Hexyl Hydrophobicit
Page 29 and 30: Accucore Phenyl-X Hydrophobicity HR
Page 31 and 32: Accucore HILIC Hydrophobicity Low p
Page 33 and 34: Accucore 150-C18 Hydrophobicity HR/
Page 35 and 36: Accucore 150-C4 Hydrophobicity HR/1
Page 37 and 38: Accucore 150-Amide-HILIC Hydrophobi
Page 39 and 40: 2-AB labelled dextran ladder mAU 12
Page 41 and 42: Accucore XL HPLC Columns Based on C
Page 43 and 44: Accucore XL C18 Hydrophobicity HR/1
Page 45 and 46: 4 µm Solid Core Particles for all
Page 47 and 48: Sensitivity S/N = 4.86 Fully porous
Page 49 and 50: Endocrine Disruptors mAU 450 400 35
Page 51 and 52: Productivity In addition to using e
Page 53 and 54: Accucore Defender Guard Cartridges
Page 55 and 56: Accucore Kits For validation of the

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