Accucore HPLC Columns - Cromlab

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Short Columns for Even Faster Separations The separating power of Accucore HPLC columns means that by using shorter column dimensions acceptable resolution can be maintained, with even greater increases in throughput and reduction in costs. 3 3.5 4 4.5 5 -100 0 0.5 1 1.5 2 2.5 Mobile phase A: water Rs=2.50 Mobile phase B: acetonitrile Gradient: Accucore RP-MS 2.6 µm 50 x 2.1 mm = 35–60 % B in 1.8 minutes Accucore RP-MS 2.6 µm Accucore RP-MS 2.6 µm 100 x 2.1 mm = 35–60 % B in 3.5 minutes 100 x 2.1 mm Flow: 400 µL/min Rs=1.51 Accucore RP-MS 2.6 µm 50 x 2.1 mm Minutes mAU Analysis Time and Solvent Savings Accucore RP-MS 2.6 µm, 50 x 2.1 mm Accucore RP-MS 2.6 µm, 100 x 2.1 mm Resolution (critical pair) 1.51 2.50 Run time (min) including gradient re-equilibration 3.00 6.00 A 50 mm column gives acceptable separation with a doubling of productivity and halving of solvent costs. 6
Higher Peak Capacity than 5 μm or 3 μm As an alternative to speeding up analysis the high resolution offered by Accucore HPLC columns can also be used to improve complex separations through an increase in peak capacity. n c Peak capacity t g Gradient time w Peak width (10% height) t g n c = 1 + ( ) w mAU fully porous 5 µm 100 x 2.1 mm fully porous 3 µm 100 x 2.1 mm Accucore RP-MS 2.6 µm 100 x 2.1 mm 0 0.5 1 1.5 2 2.5 3 Minutes Mobile phase A: water Mobile phase B: acetonitrile Gradient: Flow: Temperature: 40 °C Injection: 1 µL Detection: Analytes: 65–95 % B in 2.1 minutes 95 % B for 0.4 minutes 400 µL/min UV at 247 nm (0.1s rise time, 20 Hz) 1. Acetophenone 2. Propiophenone 3. Butyrophenone 4. Valerophenone 5. Hexanophenone 6. Heptanophenone 7. Octanophenone Peak Capacity Comparison 170 Normalized Peak Capacity 160 150 140 130 120 110 100 90 Accucore RP-MS 2.6 µm fully porous 3 µm fully porous 5 µm Accucore RP-MS 2.6 µm 158 fully porous 3 µm 132 fully porous 5 µm 100 The higher the peak capacity the more analytes can be identified. 7
Page 1 and 2: Thermo Scientific Accucore HPLC Col
Page 3 and 4: Table of Contents Particle Evolutio
Page 5 and 6: Why Core Enhanced Technology Works
Page 7: Faster than 5 μm and 3 μm Using A
Page 11 and 12: Equivalent Performance to Sub-2 μm
Page 13 and 14: Simple Method Transfer Fast HPLC is
Page 15 and 16: Instrument Optimization Accucore HP
Page 17 and 18: Long Lasting Columns Chromatographe
Page 19 and 20: Phase Characterization Accucore pha
Page 21 and 22: HR/10 AI HS IEX (2.7) C BA IEX (7.6
Page 23 and 24: Accucore C18 Hydrophobicity HR/10 L
Page 25 and 26: Accucore aQ Hydrophobicity HR/10 Lo
Page 27 and 28: Accucore Phenyl-Hexyl Hydrophobicit
Page 29 and 30: Accucore Phenyl-X Hydrophobicity HR
Page 31 and 32: Accucore HILIC Hydrophobicity Low p
Page 33 and 34: Accucore 150-C18 Hydrophobicity HR/
Page 35 and 36: Accucore 150-C4 Hydrophobicity HR/1
Page 37 and 38: Accucore 150-Amide-HILIC Hydrophobi
Page 39 and 40: 2-AB labelled dextran ladder mAU 12
Page 41 and 42: Accucore XL HPLC Columns Based on C
Page 43 and 44: Accucore XL C18 Hydrophobicity HR/1
Page 45 and 46: 4 µm Solid Core Particles for all
Page 47 and 48: Sensitivity S/N = 4.86 Fully porous
Page 49 and 50: Endocrine Disruptors mAU 450 400 35
Page 51 and 52: Productivity In addition to using e
Page 53 and 54: Accucore Defender Guard Cartridges
Page 55 and 56: Accucore Kits For validation of the

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