Accucore HPLC Columns - Cromlab
Accucore HPLC Columns - Cromlab
Accucore HPLC Columns - Cromlab
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Endocrine Disruptors<br />
mAU<br />
450<br />
400<br />
350<br />
300<br />
250<br />
200<br />
150<br />
100<br />
50<br />
0<br />
-20<br />
0<br />
∆P = 170 bar<br />
S/N = 2205<br />
PW 0.05<br />
= 0.196 min<br />
1<br />
2<br />
Fully porous C8, 5 µm<br />
1 2 3 4 5 6 7 8 9 10<br />
Time (min)<br />
3<br />
Rs = 4.12<br />
4<br />
5<br />
450<br />
∆P = 220 bar<br />
400 1<br />
<strong>Accucore</strong> XL C8, 4 µm<br />
mAU<br />
350<br />
300<br />
250<br />
200<br />
S/N = 7191<br />
PW 0.05<br />
= 0.124 min<br />
2<br />
3<br />
Rs = 5.14<br />
150<br />
100<br />
4<br />
5<br />
50<br />
0<br />
-20<br />
0<br />
1 2 3 4 5 6 7 8 9 10<br />
Time (min)<br />
Analytes<br />
1. Desethyl Atrazine<br />
2. Simazine<br />
3. Atrazine<br />
4. Diuron<br />
5. Bisphenol A<br />
<strong>Columns</strong>:<br />
Mobile phase A: water<br />
Mobile phase B: acetonitrile<br />
Gradient:<br />
Flow:<br />
<strong>Accucore</strong> XL C8 4 µm, 150 x 4.6 mm<br />
Fully porous C8 5 µm, 150 x 4.6 mm<br />
25–70 % B in 20.0 minutes<br />
70–75 % B in 0.1 minutes<br />
75–25 % B in 4.9 minutes<br />
1.5 mL/min<br />
Temperature: 25 °C<br />
Injection volume: 5 µL<br />
Detection:<br />
UV at 220 nm<br />
• 31% better resolution of critical pair<br />
• 37% narrower peaks<br />
• 226% higher sensitivity<br />
47
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