Accucore HPLC Columns - Cromlab
Accucore HPLC Columns - Cromlab
Accucore HPLC Columns - Cromlab
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Productivity<br />
In addition to using established conventional methods, the high efficiencies offered by <strong>Accucore</strong> XL <strong>HPLC</strong> columns,<br />
across a wide range of flow rates, allow methods to be optimized to reduce run times and increase productivity.<br />
1<br />
Rs = 2.47 4<br />
2 3<br />
5 6<br />
7<br />
Fully porous C18, 5 µm<br />
Standard method<br />
t = 8.62<br />
Rs = 3.13<br />
3<br />
2<br />
4<br />
5<br />
6<br />
7<br />
t = 6.56<br />
<strong>Accucore</strong> XL C18, 4 µm<br />
Standard method<br />
1<br />
Rs = 2.99<br />
2<br />
3<br />
4<br />
5<br />
6<br />
7<br />
t = 4.06<br />
<strong>Accucore</strong> XL C18, 4 µm<br />
Optimized method<br />
1<br />
0 1<br />
2 3 4 5 6 7 8 9<br />
10<br />
Minutes<br />
Column Method t R<br />
of last peak Reduction in time<br />
Fully porous C18, 5 μm Standard 8.62 min 0%<br />
<strong>Accucore</strong> XL C18, 4 μm Standard 6.56 min 24%<br />
<strong>Accucore</strong> XL C18, 4 μm Optimized 4.06 min 53%<br />
Column Dimensions:<br />
Mobile Phase A:<br />
Mobile Phase B:<br />
150 x 4.6 mm ID<br />
water<br />
acetonitrile<br />
Standard Method<br />
Optimized Method<br />
Gradient: 35–60% B in 10 minutes 35–60% B in 4 minutes<br />
Flow: 1.0 mL/min 1.3 mL/min<br />
Temperature: 30 °C<br />
Injection: 5 µL<br />
Detection:<br />
Analytes: 1. Uracil (t 0<br />
)<br />
2. Tebuthiuron<br />
3. Metoxuron<br />
4. Monuron<br />
5. Chlorotoluron<br />
6. Diuron<br />
7. Linuron<br />
UV at 247 nm (0.1 s rise time, 20 Hz)<br />
Run time reduced by over a third with an improvement in performance.<br />
49
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