Accucore HPLC Columns - Cromlab

Accucore C30
Hydrophobicity
HR/10
Low
pH Range
High
AI
HS
0
Pore Size
2 to 8
14
IEX (2.7)
SS
0
Carbon Load (%)
150 Å
300
C
HBC
0
5
25
Particle Size 2.6 µm
USP L62
BA IEX (7.6)
• Ideal for separation of hydrophobic, long alkyl chain compounds
• High shape selectivity for structurally related isomers
• Excellent aqueous-compatibility
Accucore C30 offers high shape selectivity for hydrophobic, long chain, structurally related isomers, for example
carotenoids and steroids. This is a different form of shape selectivity from that measured in the SS phase
characterisation test.
It is also an excellent alternative to normal-phase columns for lipid analysis. The optimized bonding density of the
long alkyl chains facilitated by a wider pore diameter particle result in a phase that is stable even in highly aqueous
mobile phases.
Vitamin K isomers
mAU
250
200
150
100
1
1
2+2’
Fused Core C18
Accucore C30
Columns:
Accucore C30 2.6 µm, 100 x 3.0 mm
Fused Core C18, 100 x 3.0 mm
Mobile phase: methanol: 2 mM ammonium acetate, 98:2
Flow:
650 µL/min
Temperature: 20 °C
Injection: 5 µL
Detection:
UV at 250 nm
Accucore C30 shows better separation for vitamin K1 isomers than the C18
column.
50
2
2’
0
-50
0.00 1.00 2.00 3.00 4.00 5.00 6.00
Minutes
Chromatogram showing the separation of Vitamin K compounds
1-Vitamin K2, 2-Vitamin K1 (trans isomer), 2’-Vitamin K1 (cis isomer)
28