Accucore HPLC Columns - Cromlab

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Accucore PFP Hydrophobicity HR/10 Low pH Range High AI HS 0 2 to 8 14 Pore Size IEX (2.7) SS 0 80Å 300 Carbon Load (%) 0 5 25 C HBC Particle Size 2.6 µm USP L43 BA IEX (7.6) • Alternative selectivity to C18 • Extra retention for halogenated species • Unique selectivity for non-halogenated polar compounds Introduction of fluorine groups into the Accucore PFP (pentafluorophenyl) stationary phase Accucore causes XL significant C18 changes in solute-stationary phase interactions. This can lead to extra retention and selectivity for positional isomers of halogenated compounds. PFP Columns are also well suited to the selective analysis of non-halogenated compounds, in particular polar compounds containing hydroxyl, carboxyl, nitro, or other polar groups. High selectivity is often most apparent when the functional groups are located on an aromatic or other rigid ring system. Positional Isomers mAU 70 60 50 40 30 20 10 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 Minutes 10 11 12 13 14 Column: Accucore PFP 2.6 µm, 50 x 2.1 mm Mobile phase A: water + 0.1 % formic acid Mobile phase B: acetonitrile + 0.1 % formic acid Gradient: Flow: Temperature: 50 °C Injection: 2 µL Detection: 15–30 % B in 7 minutes 600 µL/min UV at 270 nm Analytes: 1. 3,4-Dimethoxyphenol 2. 2,6-Dimethoxyphenol 3. 2,6-Difluorophenol 4. 3,5-Dimethoxyphenol 5. 2,4-Difluorophenol 6. 2,3-Difluorophenol 7. 3,4-Difluorophenol 8. 3,5-Dimethylphenol 9. 2,6-Dimethylphenol 10. 2,6-Dichlorophenol 11. 4-Chloro-3-Methylphenol 12. 4-Chloro-2-Methylphenol 13. 3,4-Dichlorophenol 14. 3,5-Dichlorophenol 26
Accucore Phenyl-X Hydrophobicity HR/10 Low pH Range High AI HS 0 Pore Size 2 to 8 14 IEX (2.7) SS 0 80 Å Carbon Load (%) 300 C HBC 0 6 25 Particle Size 2.6 µm BA IEX (7.6) • Unique reversed-phase shape selectivity • Enhanced selectivity for aromatic compounds • Compatible with highly aqueous mobile phases • Robust, high-efficiency, low column bleed The proprietary Accucore Phenyl-X alkyl aromatic bonded phase provides a unique selectivity when compared to other reversed phase materials such as C18 or Phenyl. Phenyl-X exhibits particularly high aromatic selectivity. The advanced design of the bonded phase makes it compatible with highly aqueous mobile phases and robust, demonstrating very low bleed. Estrogens mAU 120 100 80 60 40 20 0 1 2 4 3 Fused Core C18 Columns: Mobile phase: Flow: Accucore Phenyl-X 2.6 µm, 100 x 2.1 mm Fused Core C18, 100 x 2.1 mm 15:40:45 (v/v) acetonitrile: methanol : water 400 µL/min Temperature: 40 °C Injection: 1 µL Detection: Wash solvent: UV at 220 nm Same as mobile phase mAU 0.0 0.5 1.0 2.5 2.0 2.5 3.0 3.5 4.0 5.5 6.0 Minutes 150 1 125 100 2 3 2.04 75 4 50 25 Accucore Phenyl-X 0 0.0 0.5 1.0 2.5 2.0 2.5 3.0 3.5 4.0 5.5 6.0 Minutes 1. Estriol (E3) OH H 3 C OH H H H HO 3. Estrone (E1) OH H 3 C H H H HO 2. Estradiol (E2) OH H 3 C H H H HO 4. Ethynylestradiol OH H 3 C CH H H H HO 27
Page 1 and 2: Thermo Scientific Accucore HPLC Col
Page 3 and 4: Table of Contents Particle Evolutio
Page 5 and 6: Why Core Enhanced Technology Works
Page 7 and 8: Faster than 5 μm and 3 μm Using A
Page 9 and 10: Higher Peak Capacity than 5 μm or
Page 11 and 12: Equivalent Performance to Sub-2 μm
Page 13 and 14: Simple Method Transfer Fast HPLC is
Page 15 and 16: Instrument Optimization Accucore HP
Page 17 and 18: Long Lasting Columns Chromatographe
Page 19 and 20: Phase Characterization Accucore pha
Page 21 and 22: HR/10 AI HS IEX (2.7) C BA IEX (7.6
Page 23 and 24: Accucore C18 Hydrophobicity HR/10 L
Page 25 and 26: Accucore aQ Hydrophobicity HR/10 Lo
Page 27: Accucore Phenyl-Hexyl Hydrophobicit
Page 31 and 32: Accucore HILIC Hydrophobicity Low p
Page 33 and 34: Accucore 150-C18 Hydrophobicity HR/
Page 35 and 36: Accucore 150-C4 Hydrophobicity HR/1
Page 37 and 38: Accucore 150-Amide-HILIC Hydrophobi
Page 39 and 40: 2-AB labelled dextran ladder mAU 12
Page 41 and 42: Accucore XL HPLC Columns Based on C
Page 43 and 44: Accucore XL C18 Hydrophobicity HR/1
Page 45 and 46: 4 µm Solid Core Particles for all
Page 47 and 48: Sensitivity S/N = 4.86 Fully porous
Page 49 and 50: Endocrine Disruptors mAU 450 400 35
Page 51 and 52: Productivity In addition to using e
Page 53 and 54: Accucore Defender Guard Cartridges
Page 55 and 56: Accucore Kits For validation of the

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