Accucore HPLC Columns - Cromlab

Accucore 150-Amide-HILIC
Hydrophobicity
Low
pH Range
0
Pore Size
2 to 8
High
14
HILIC
0 150 Å
300
Particle Size 2.6 µm
• Amide phase bonded onto 150 Å pore diameter solid core particles
• High retention of a broad range of hydrophilic analytes in HILIC mode
• Recommended for hydrophilic biomolecules such as glycans
Accucore 150-Amide-HILIC is designed for the separation of hydrophilic biomolecules in HILIC mode.
The amide bonded phases provide strong hydrogen bonding interaction between the stationary phase and the
analytes, resulting in unique selectivity compared to other HILIC phases. Combined with larger pore size of the solid
core particles, Accucore 150-Amide-HILIC is well suited for separating a variety of hydrophilic molecules, including
carbohydrates and peptides. As a result the Accucore 150-Amide-HILIC is an excellent choice for glycan separations.
Glycan Ladder
Counts
1200000
1100000
1000000
900000
800000
700000
600000
500000
400000
300000
200000
100000
0
-100000
-200000
1
2
3
A
(A) 2 µL injection of sample, where 11 glycans were separated.
4
30 35 40 45
Minutes
(B) 5 µL injection of sample, zoomed-in to the later part of the gradient rise.
A further 10 glycans were detected.
5
350000
300000
250000
200000
150000
100000
50000
6
0
10
7
5 10 15 20 25 30 35 40 45
8
Minutes
9
10 11
B
11
12
13
14
15
16 17 18 19 20 21
Column:
Mobile phase A:
Accucore 150-Amide-HILIC, 2.6 µm,
100 x 2.1 mm
acetonitrile
Mobile phase B: 50 mM ammonium formate, pH 4.5
Gradient:
Flow:
Backpressure at starting
conditions:
Run time:
20–50 % B in 40.0 minutes
50 % B for 5.0 minutes
50–20 % B in 0.5 minutes
50 % B for 4.5 minutes
500 µL/min
110 bar
50 minutes
Temperature: 60 °C
Injection:
Injection wash solvent:
Fluorescence detector
acquisition parameters:
2 µL to 5 µL of sample.
80:20 (v/v) acetonitrile:water.
330 nm excitation wavelength;
420 nm emission wavelength; acquisition start
after 3 min from gradient start.
35