Accucore HPLC Columns - Cromlab

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Accucore HPLC columns are also stable at pH >10 Capacity Factor mAU 220 180 Injection 400 140 100 Injection 220 60 20 Injection 90 0 0 1 2 3 4 5 6 7 8 9 10 11 Minutes 50 45 40 35 30 25 20 15 10 5 0 5000 10000 15000 20000 25000 30000 35000 Column Volumes Column: Accucore C18 2.6 µm, 100 x 2.1 mm Mobile phase A: water + 0.1 % ammonia Mobile phase B: methanol + 0.1 % ammonia Gradient: Flow: Temperature: 30 °C Injection: 1 µL Detection: 15 % B for 1.0 minutes 15–100 % B in 7.0 minutes 100 % B for 3.00 minutes 100–15 % B in 0.20 minutes 15 % B for 4.80 minutes 400 µL/min UV at 254 nm (0.1s rise time, 20 Hz) Analytes: 1. Uracil (t 0 ) 2. 4-Chlorocinnamic acid 3. Procainamide 4. 4-Pentylbenzoic Acid 5. N-Acetylprocainamide 6. Di-isopropyl phthalate 7. Di-n-propyl phthalate And also stable at elevated temperature mAU 150 100 50 0 Injection 400 Injection 130 Injection 20 0 0.2 0.6 1 1.4 1.8 2.2 2.6 3 Minutes Column: Mobile phase: Flow: Temperature: 70 °C Accucore C18 2.6 µm, 100 x 2.1 mm 35:65 (v/v) water/methanol 400 µL/min Injection: 1.5 µL Detection: Analytes: UV at 254 nm (0.1s rise time, 20 Hz) 1. Theophylline (t 0 )/Caffeine 2. Phenol 3. Butylbenzene 4. o-Terphenyl 5. Pentylbenzene/Triphenylene 9 8 Capacity Factor 7 6 5 4 3 2 1 0 2000 4000 6000 Column Volumes 8000 10000 16
Phase Characterization Accucore phases are characterized using three tests based on the Tanaka testing protocols 1 . This detailed phase characterization allows the retentivity, selectivity and secondary interactions demonstrated by HPLC packing materials under specified conditions to be objectively compared. T1: Hydrophobic Interactions Parameter Term HR Hydrophobic Retention Retention of compounds based on their hydrophobicity k' HS Hydrophobic Selectivity Separation of compounds that have similar structure, but differ slightly in hydrophobicity α SS Steric Selectivity Separation of compounds that have similar structure, but differ in shape α HBC Hydrogen Bonding Capacity Separation related to degree of end capping α T2: Secondary Interactions Under Neutral pH Parameter Term BA Base Activity Peak shape for basic analytes resulting from total silanol activity (all dissociated at pH 7.6) t f C Chelation Peak shapes for chelating analytes resulting from silica metal content t f IEX(7.6) Ion Exchange Capacity (pH 7.6) Separation between basic and neutral compounds resulting from total silanol activity (all dissociated at pH 7.6) α T3: Secondary Interactions Under Acidic pH Parameter Term AI Acid Interaction Interactions resulting in poor peak shape for acidic analytes t f IEX(2.7) Ion Exchange Capacity (pH 2.7) Separation between basic and neutral compounds resulting from acidic silanol activity α The results of the phase characterizations are shown in the radar plots used in this guide. 1. K. Kimata, K. Iwaguchi, S. Onishi, K. Jinno, R. Eksteen, K. Hosoya, M. Arki, N. Tanaka, J. Chromatogr. Sci. 27 (1989) 721 17
Page 1 and 2: Thermo Scientific Accucore HPLC Col
Page 3 and 4: Table of Contents Particle Evolutio
Page 5 and 6: Why Core Enhanced Technology Works
Page 7 and 8: Faster than 5 μm and 3 μm Using A
Page 9 and 10: Higher Peak Capacity than 5 μm or
Page 11 and 12: Equivalent Performance to Sub-2 μm
Page 13 and 14: Simple Method Transfer Fast HPLC is
Page 15 and 16: Instrument Optimization Accucore HP
Page 17: Long Lasting Columns Chromatographe
Page 21 and 22: HR/10 AI HS IEX (2.7) C BA IEX (7.6
Page 23 and 24: Accucore C18 Hydrophobicity HR/10 L
Page 25 and 26: Accucore aQ Hydrophobicity HR/10 Lo
Page 27 and 28: Accucore Phenyl-Hexyl Hydrophobicit
Page 29 and 30: Accucore Phenyl-X Hydrophobicity HR
Page 31 and 32: Accucore HILIC Hydrophobicity Low p
Page 33 and 34: Accucore 150-C18 Hydrophobicity HR/
Page 35 and 36: Accucore 150-C4 Hydrophobicity HR/1
Page 37 and 38: Accucore 150-Amide-HILIC Hydrophobi
Page 39 and 40: 2-AB labelled dextran ladder mAU 12
Page 41 and 42: Accucore XL HPLC Columns Based on C
Page 43 and 44: Accucore XL C18 Hydrophobicity HR/1
Page 45 and 46: 4 µm Solid Core Particles for all
Page 47 and 48: Sensitivity S/N = 4.86 Fully porous
Page 49 and 50: Endocrine Disruptors mAU 450 400 35
Page 51 and 52: Productivity In addition to using e
Page 53 and 54: Accucore Defender Guard Cartridges
Page 55 and 56: Accucore Kits For validation of the

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