Accucore HPLC Columns - Cromlab

More documents

Recommendations

Info

Accucore C30 Hydrophobicity HR/10 Low pH Range High AI HS 0 Pore Size 2 to 8 14 IEX (2.7) SS 0 Carbon Load (%) 150 Å 300 C HBC 0 5 25 Particle Size 2.6 µm USP L62 BA IEX (7.6) • Ideal for separation of hydrophobic, long alkyl chain compounds • High shape selectivity for structurally related isomers • Excellent aqueous-compatibility Accucore C30 offers high shape selectivity for hydrophobic, long chain, structurally related isomers, for example carotenoids and steroids. This is a different form of shape selectivity from that measured in the SS phase characterisation test. It is also an excellent alternative to normal-phase columns for lipid analysis. The optimized bonding density of the long alkyl chains facilitated by a wider pore diameter particle result in a phase that is stable even in highly aqueous mobile phases. Vitamin K isomers mAU 250 200 150 100 1 1 2+2’ Fused Core C18 Accucore C30 Columns: Accucore C30 2.6 µm, 100 x 3.0 mm Fused Core C18, 100 x 3.0 mm Mobile phase: methanol: 2 mM ammonium acetate, 98:2 Flow: 650 µL/min Temperature: 20 °C Injection: 5 µL Detection: UV at 250 nm Accucore C30 shows better separation for vitamin K1 isomers than the C18 column. 50 2 2’ 0 -50 0.00 1.00 2.00 3.00 4.00 5.00 6.00 Minutes Chromatogram showing the separation of Vitamin K compounds 1-Vitamin K2, 2-Vitamin K1 (trans isomer), 2’-Vitamin K1 (cis isomer) 28
Accucore HILIC Hydrophobicity Low pH Range Pore Size High 0 2 to 8 14 HILIC 0 80Å 300 Particle Size 2.6 µm USP L3 • Enhanced retention of polar and hydrophilic analytes • Alternative selectivity to C18 without ion-pair or derivatization Analyte properties that govern retention with Accucore HILIC phase are acidity/basicity, which determines hydrogen bonding, and polarizability which determines dipole-dipole interactions. The highly organic mobile phases used with Accucore HILIC phase ensure efficient desolvation in ESI MS detection, which in turn leads to improved sensitivity. Catecholamines mAU 140 120 100 80 60 40 20 0 1 2 3 4 5 6 7 0 0.5 1 1.5 2 2.5 3 3.5 4 Minutes Column: Mobile phase: Flow: Temperature: 40 °C Injection: 5 µL Detection: Backpressure: Analytes: Accucore HLIC 2.6 µm, 150 x 4.6 mm 85:15 (v/v) acetonitrile/100mM ammonium formate, pH 3.2 2000 µL/min UV at 280 nm 157 bar 1. Catechol 2. 5-HIAA 3. DOPAC 4. Serotonin 5. L-tyrosine 6. Dopamine 7. L-DOPA 29
Page 1 and 2: Thermo Scientific Accucore HPLC Col
Page 3 and 4: Table of Contents Particle Evolutio
Page 5 and 6: Why Core Enhanced Technology Works
Page 7 and 8: Faster than 5 μm and 3 μm Using A
Page 9 and 10: Higher Peak Capacity than 5 μm or
Page 11 and 12: Equivalent Performance to Sub-2 μm
Page 13 and 14: Simple Method Transfer Fast HPLC is
Page 15 and 16: Instrument Optimization Accucore HP
Page 17 and 18: Long Lasting Columns Chromatographe
Page 19 and 20: Phase Characterization Accucore pha
Page 21 and 22: HR/10 AI HS IEX (2.7) C BA IEX (7.6
Page 23 and 24: Accucore C18 Hydrophobicity HR/10 L
Page 25 and 26: Accucore aQ Hydrophobicity HR/10 Lo
Page 27 and 28: Accucore Phenyl-Hexyl Hydrophobicit
Page 29: Accucore Phenyl-X Hydrophobicity HR
Page 33 and 34: Accucore 150-C18 Hydrophobicity HR/
Page 35 and 36: Accucore 150-C4 Hydrophobicity HR/1
Page 37 and 38: Accucore 150-Amide-HILIC Hydrophobi
Page 39 and 40: 2-AB labelled dextran ladder mAU 12
Page 41 and 42: Accucore XL HPLC Columns Based on C
Page 43 and 44: Accucore XL C18 Hydrophobicity HR/1
Page 45 and 46: 4 µm Solid Core Particles for all
Page 47 and 48: Sensitivity S/N = 4.86 Fully porous
Page 49 and 50: Endocrine Disruptors mAU 450 400 35
Page 51 and 52: Productivity In addition to using e
Page 53 and 54: Accucore Defender Guard Cartridges
Page 55 and 56: Accucore Kits For validation of the

Accucore HPLC Columns - Cromlab

Create successful ePaper yourself

Delete template?

Save as template?