Accucore HPLC Columns - Cromlab

Simple Method Transfer
Fast HPLC is often performed using lower volume columns.
A few simple steps are required to transfer a method to a lower volume Accucore HPLC column.
Method Transfer Tool
A convenient method transfer tool is available at the Chromatography Resource Center
www.thermoscientific.com/crc
• Adjust Flow Rate
Keep linear velocity constant between original and new method, taking into account
particle size and geometry
• Adjust Injection Volume
Keep the ratio of injection volume to column volume constant
• Adjust Gradient Profile
Keep the number of column volumes constant for each gradient segment
mAU
fully porous 5 μm
150 x 4.6 mm
Accucore RP-MS 2.6 µm
100 x 2.1 mm
0 1 2 3 4 5 6 7 8 9 10
Minutes
Mobile phase A: water
Mobile phase B: acetonitrile
Gradient: Accucore RP-MS 2.6 µm
100 x 2.1 mm = 35–60 % B in 3.5 minutes
fully porous 5 µm
150 x 4.6 mm = 35–60 % B in 10.0 minutes
Flow: Accucore RP-MS 2.6 µm
100 x 2.1 mm = 400 µL/min
fully porous 5 µm
150 x 4.6 mm = 1000 µL/min
Injection: Accucore RP-MS 2.6 µm
100 x 2.1 mm = 1 µL
fully porous 5 µm
150 x 4.6 mm = 5 µL
Temperature: 30 °C
Detection:
Analytes:
UV at 247 nm (0.1s rise time, 20 Hz)
1. Tebuthiuron
2. Metoxuron
3. Monuron
4. Chlorotoluron
5. Diuron
6. Linuron
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