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Accucore HPLC Columns - Cromlab

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<strong>Accucore</strong> C18<br />

Hydrophobicity<br />

HR/10<br />

Low<br />

pH Range<br />

High<br />

AI<br />

HS<br />

0 1 to 11<br />

14<br />

Pore Size<br />

IEX (2.7)<br />

SS<br />

0 80Å<br />

300<br />

Carbon Load (%)<br />

0 9<br />

25<br />

C<br />

HBC<br />

Particle Size 2.6 µm USP L1<br />

BA IEX (7.6)<br />

• Optimum retention of non-polar compounds<br />

• Hydrophobic interaction mechanism<br />

• Separates a broad range of analytes<br />

The carbon loading of <strong>Accucore</strong> C18 phase provides high retention of non-polar analytes <strong>Accucore</strong> via a predominantly XL C18<br />

hydrophobic interaction mechanism.<br />

The highly retentive nature of <strong>Accucore</strong> C18 phase means that it can be used to separate a broad range of analytes.<br />

Triazines<br />

mAU<br />

100<br />

90<br />

80<br />

70<br />

60<br />

50<br />

40<br />

30<br />

20<br />

10<br />

0<br />

1<br />

2<br />

3<br />

0.0 0.5 1.0 1.5 2.0 2.5 3.0<br />

Minutes<br />

4<br />

5<br />

6<br />

Column:<br />

Mobile phase A: water<br />

Mobile phase B: acetonitrile<br />

Gradient:<br />

Flow:<br />

Temperature: 25 °C<br />

Injection: 2 µL<br />

Detection:<br />

Backpressure:<br />

Analytes:<br />

<strong>Accucore</strong> C18 2.6 µm, 50 mm x 2.1 mm<br />

35 % B for 1.0 minute<br />

35–70 % B in 1.5 minutes<br />

600 µL/min<br />

UV at 280 nm<br />

298 bar<br />

1. Simazine<br />

2. Simetryn<br />

3. Atrazine<br />

4. Ametryn<br />

5. Propazine<br />

6. Prometryn<br />

21

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