Accucore HPLC Columns - Cromlab

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High Peak Capacity Higher peak capacities facilitate increased peptide identifications. Accucore 150-C18 provides much narrower peak widths, therefore significantly higher peak capacity than a column packed with < 2 µm wide pore fully porous C18. Accucore 150-C18 Backpressure: 175 bar conventional solid core C18 Backpressure: 170 bar Peak capacity 150.00 140.00 130.00 120.00 110.00 100.00 90.00 80.00 70.00 60.00 Accucore 150 - C18 Peak Capacity Comparison Conventional Solid Core C18 < 2 μm Wide Pore Fully Porous C18 uAU 100000 50000 0 -50000 < 2 µm wide pore fully porous C18 Backpressure: 320 bar 2 4 6 8 10 12 14 Time (min) n c Peak capacity t g n c = t 1 + ( g ) w Gradient time w Average peak width 10% height Reproducible Separations Precision of retention times is critical for reliable analysis. The Accucore 150-C18 column exhibits excellent retention time reproducibility. 13.0 13.5 14.0 Protein Mean Retention Time (min) % RSD Insulin 13.58 0.11 Data from six injections. 32
Accucore 150-C4 Hydrophobicity HR/10 Low pH Range AI HS 0 2 to 9 14 Pore Size IEX (2.7) SS 0 150 Å 300 Carbon Load (%) 0 2 25 C HBC Particle Size 2.6 µm USP L26 BA IEX (7.6) • Significantly lower hydrophobic retention than C18 • Ideal for retention of proteins and larger peptides Intact Protein Separation 130000 120000 110000 100000 5 90000 uAU 80000 70000 60000 50000 40000 30000 20000 10000 0 -10000 1 2 * 3 4 6 Column: Accucore 150-C4 2.6 µm, 100 x 2.1 mm Mobile phase A: 0.1 % TFA in 30:70 acetonitrile:water Mobile phase B: 0.1 % TFA in 98:2 acetonitrile:water Gradient: Flow: 0–30 % B in 8.0 minutes 30–95 % B in 2.0 minutes 95 % B for 1.0 minute 95–0 % B in 0.1 minutes 0 % B for 4.0 minutes 400 µL/min Temperature: 40 °C Injection: Detection: 2 µL of 10 pmol/µL solution UV (214 and 280 nm) 0 1 2 3 4 5 6 7 8 9 10 Time (min) Peak Number Retention Time (min) Protein MW (kDa) Concentration (µg/mL) 1 1.54 Insulin 6 40 2 2.66 Cytochrome C 12 80 3 4.42 Lysozyme 14 100 4 7.38 Myoglobin 18 120 5 7.88 Carbonic anhydrase 30 200 6 7.88 Ovalbumin 45 300 * Carbonic anhydrase impurity 33
Page 1 and 2: Thermo Scientific Accucore HPLC Col
Page 3 and 4: Table of Contents Particle Evolutio
Page 5 and 6: Why Core Enhanced Technology Works
Page 7 and 8: Faster than 5 μm and 3 μm Using A
Page 9 and 10: Higher Peak Capacity than 5 μm or
Page 11 and 12: Equivalent Performance to Sub-2 μm
Page 13 and 14: Simple Method Transfer Fast HPLC is
Page 15 and 16: Instrument Optimization Accucore HP
Page 17 and 18: Long Lasting Columns Chromatographe
Page 19 and 20: Phase Characterization Accucore pha
Page 21 and 22: HR/10 AI HS IEX (2.7) C BA IEX (7.6
Page 23 and 24: Accucore C18 Hydrophobicity HR/10 L
Page 25 and 26: Accucore aQ Hydrophobicity HR/10 Lo
Page 27 and 28: Accucore Phenyl-Hexyl Hydrophobicit
Page 29 and 30: Accucore Phenyl-X Hydrophobicity HR
Page 31 and 32: Accucore HILIC Hydrophobicity Low p
Page 33: Accucore 150-C18 Hydrophobicity HR/
Page 37 and 38: Accucore 150-Amide-HILIC Hydrophobi
Page 39 and 40: 2-AB labelled dextran ladder mAU 12
Page 41 and 42: Accucore XL HPLC Columns Based on C
Page 43 and 44: Accucore XL C18 Hydrophobicity HR/1
Page 45 and 46: 4 µm Solid Core Particles for all
Page 47 and 48: Sensitivity S/N = 4.86 Fully porous
Page 49 and 50: Endocrine Disruptors mAU 450 400 35
Page 51 and 52: Productivity In addition to using e
Page 53 and 54: Accucore Defender Guard Cartridges
Page 55 and 56: Accucore Kits For validation of the

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