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Special Issue Survey of Cetaceans in Captive Care

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6. Life Support Systems 361contact chamber. Microorganisms, such as bacteria,algae, yeast, and viruses, are killed or <strong>in</strong>activatedby the UV radiation produced by the UV lamp thatpenetrates the microorganism’s cell wall and membrane,destroy<strong>in</strong>g the <strong>in</strong>ner material. These microorganismsrequire a precise wavelength and duration<strong>of</strong> irradiation to be destroyed; otherwise, they canresist, repair, and regrow (M<strong>of</strong>idi et al., 2002). UVsterilisation is a po<strong>in</strong>t-contact process. Efficiencydepends on the time <strong>of</strong> contact; therefore, the speed<strong>of</strong> the flow is crucial. UV sterilisation efficiency isimpeded by turbidity; the higher the number <strong>of</strong> suspendedparticles <strong>in</strong> the water, the less light penetration(Spotte, 1991). The precise UV dosage will bedeterm<strong>in</strong>ed by the quality <strong>of</strong> water and the type <strong>of</strong>microorganisms targeted. UV dose is recorded as aproduct <strong>of</strong> irradiance (milliwatts per square centimetre)and time (second), which provides units <strong>of</strong>milliJoules per square centimetre (mJ/cm 2 ) (M<strong>of</strong>idiet al., 2002). Low-pressure lamps are preferred asthey have a longer life and are more economical toreplace. Recommended dosage is between 20 and35 mJ/cm 2 (calculated at the end <strong>of</strong> lamp life) ata wave length <strong>of</strong> 254 nanometer (nm). UV lightsterilisation is not the most efficient system forlarge volumes (Spotte, 1991); it is also one <strong>of</strong> theless economical (Boness, 1996); however, it issafer <strong>in</strong> comparison with other means <strong>of</strong> sterilisation<strong>in</strong> terms <strong>of</strong> potential production <strong>of</strong> harmfulbyproducts.I recommend that a newly <strong>in</strong>stalled systems berun for several days to several weeks before <strong>in</strong>troduc<strong>in</strong>ganimals <strong>in</strong>to the pool to ensure that thereare no toxicity concerns, leakage, or loose materialsfrom the LSS.In conclusion, there is not one solution that hasall the advantages. The survey showed that newLSSs tend to be more complex and that a comb<strong>in</strong>ation<strong>of</strong> techniques is the most efficient solution.Nowadays, it is common to see an LSS equippedwith high-pressure sand filters as the basicmechanical <strong>in</strong>stallation. This is used <strong>in</strong> comb<strong>in</strong>ationwith either a high dosage <strong>of</strong> ozone or prote<strong>in</strong>skimmers, comb<strong>in</strong>ed with a lower dosage <strong>of</strong>ozone. This also is associated with activated carbonfilters or denitrification filters on side-streams,sometimes a side-stream treated by UV, and <strong>of</strong>tena small dosage <strong>of</strong> chlor<strong>in</strong>e as a residual oxidant <strong>in</strong>bulk-fluid to prolong oxidation capacity and controlalgal growth. This comb<strong>in</strong>ation <strong>of</strong> techniquesallows all <strong>of</strong> the different and extremely complexreactions occurr<strong>in</strong>g <strong>in</strong> cetacean pool water purificationto be tackled with more subtlety.Water Flow and Dra<strong>in</strong>ageFlow mechanics <strong>in</strong> pools are a delicate and complicatedmatter, and they are difficult to summarise.Figure 6.8. UV unit (Photograph from I. Smit)Water circulation depends ma<strong>in</strong>ly on the volume <strong>of</strong>water and the topography and depth <strong>of</strong> the pool. Ingeneral, water flows <strong>in</strong>to the pool through several<strong>in</strong>let pipes located <strong>in</strong> the upper section, and sumpslocated at the bottom <strong>of</strong> the pool collect water tooutlet pipes. Both <strong>in</strong>let and outlet pipes shouldbe placed <strong>in</strong> such a way that they leave no “deadcorner” <strong>in</strong> the pool. The survey <strong>in</strong>dicated that itis sometimes obvious that such pockets <strong>of</strong> poorlyagitated water exist when leaves, food debris, anddirt regularly accumulate at the bottom.Water <strong>in</strong>let pipes also can be located all aroundthe perimeter <strong>of</strong> the pool, close to the water surface.This system is found <strong>in</strong> semi-natural poolswhere pipes can be hidden beh<strong>in</strong>d rocks and aredesigned to distribute water evenly.Air entrapment <strong>in</strong> the water column is acommon problem <strong>in</strong> a water circulation system.High turbidity levels <strong>in</strong>crease air entrapment assuspended solids b<strong>in</strong>d with air bubbles and preventthem from ascend<strong>in</strong>g to the surface and escap<strong>in</strong>g.It is caused by a high water flow rate that producesturbulence <strong>in</strong> some parts <strong>of</strong> the circulation system.It does not cause an immediate health threat to theanimals, but it can severely disrupt their echolocationabilities (Fasik, 1991).Water turnover rate depends on various factorssuch as the volume <strong>of</strong> water to treat or replace,the number <strong>of</strong> animals, the water temperature, andthe sterilisation system. The survey <strong>in</strong>dicated that

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