360 Couquiaud<strong>in</strong>stallation to warn aga<strong>in</strong>st the presence <strong>of</strong> ozone<strong>in</strong> the surround<strong>in</strong>g atmosphere. The ozone <strong>in</strong>stallationcan be switched <strong>of</strong>f when the measured valueexceeds 0.1 ppm.The chemistry <strong>of</strong> ozone is <strong>in</strong>fluenced by thepresence <strong>of</strong> bromide; therefore, it is modifiedma<strong>in</strong>ly by seawater, artificial seawater, and freshwater,which conta<strong>in</strong> this element. Ozonation <strong>of</strong>seawater oxidises bromide to brom<strong>in</strong>e and, ultimately,as bromate (as described similarly withchlor<strong>in</strong>e above). Also like chlor<strong>in</strong>e, <strong>in</strong> the presence<strong>of</strong> humic acid, it can yield toxic compounds,possibly at a higher level than chlor<strong>in</strong>ation.Aga<strong>in</strong>, the very limited amount <strong>of</strong> <strong>in</strong>gested poolwater by cetaceans makes it most likely harmless.Ozonation <strong>in</strong> the presence <strong>of</strong> bromide is lessefficient and <strong>in</strong>creases ozone demand, but it als<strong>of</strong>orms persistent oxidants that serve as weak bulkfluidsterilis<strong>in</strong>g agents (Spotte, 1991).Chlor<strong>in</strong>ation <strong>in</strong> small dosages can be used <strong>in</strong>conjunction with ozonation to prolong the sterilisationeffect or limit algal growth. The use <strong>of</strong> ozoneis compatible with prote<strong>in</strong> skimmers (see comb<strong>in</strong>edtechniques below). One <strong>of</strong> the greatest advantages<strong>of</strong> ozone, compared to chlor<strong>in</strong>e, is that fish andcrustaceans can survive <strong>in</strong> ozonated water (if thereis no hypobromic acid). This creates a richer andpotentially more <strong>in</strong>teractive environment for theanimals and a visually more attractive display tothe visitors. The animals also can help ma<strong>in</strong>ta<strong>in</strong>a cleaner environment by graz<strong>in</strong>g on the algae.With the <strong>in</strong>creas<strong>in</strong>g popularity <strong>of</strong> ozone systems tosterilise mar<strong>in</strong>e mammal pools as well as potablewater, ozonators are becom<strong>in</strong>g cheaper, safer, andmore reliable. Ozone is considered one <strong>of</strong> the beststerilis<strong>in</strong>g options for dolph<strong>in</strong>aria at present.Biological PurificationBiological filtration is the process by which wasteproducts, pr<strong>in</strong>cipally ammonia, are broken downby bacteria us<strong>in</strong>g them as a food source. Systems,such as trickl<strong>in</strong>g filters, seldom have been used <strong>in</strong>oceanaria; therefore, we will not exam<strong>in</strong>e them.Biological filtration ma<strong>in</strong>ly is used <strong>in</strong> the denitrificationprocess to remove accumulat<strong>in</strong>g nitrate<strong>in</strong> a system by transform<strong>in</strong>g it to nitrogen gas.The denitrification process is <strong>in</strong>stalled on a sidestreamto treat a percentage <strong>of</strong> the water each day.There are two k<strong>in</strong>ds <strong>of</strong> biological denitrification:(1) heterotrophic and (2) autotrophic. Heterotrophicdenitrification is based on anaerobic bacteriaus<strong>in</strong>g nitrite and nitrate as a food source and ethanolor methanol as carbon substrate (Hignetteet al., 1997). This method requires a very slow watercirculation speed <strong>in</strong> the filter (0.2 to 2 m/h) and ahigh consumption <strong>of</strong> carbon substrate, mak<strong>in</strong>g itmore appropriate for small volumes <strong>in</strong> aquariologythan for mar<strong>in</strong>e mammal water treatment.Figure 6.6. Ozone control cab<strong>in</strong>et (Photograph from I. Smit)Figure 6.7. Small ozone contact chamberAutotrophic denitrification is based upon a sulphuroxidis<strong>in</strong>g bacterium (Thiobacillus denitrificans)<strong>in</strong> tanks filled with sulphur balls (1 to 4 mmdiameter) and calcium carbonate. Calcium carbonateis present to remove the sulphates produced andto balance the acidification <strong>of</strong> the pH <strong>in</strong>duced by thedenitrification (Hignette et al., 1997). This techniquesusta<strong>in</strong>s much higher filtration speed (2 to 9 m/h)and is more tolerant to dissolved oxygen than theheterotrophic denitrification method. Denitrificationis not yet common <strong>in</strong> mar<strong>in</strong>e mammal LSSs, but itis ga<strong>in</strong><strong>in</strong>g <strong>in</strong> popularity, and the autotrophic methodis certa<strong>in</strong>ly the most appropriate.UV-Light IrradiationUltra-Violet (UV) light irradiation is neither amechanical, chemical, nor biological process. It ismore <strong>of</strong> a physical process, and it is quite widelyused—ma<strong>in</strong>ly for small volumes or when othermar<strong>in</strong>e organisms, such as fish and <strong>in</strong>vertebrates,which do not tolerate the presence <strong>of</strong> a residual dis<strong>in</strong>fectantlike chlor<strong>in</strong>e, are present. It also can beused for the destruction <strong>of</strong> residual ozone after the
6. Life Support Systems 361contact chamber. Microorganisms, such as bacteria,algae, yeast, and viruses, are killed or <strong>in</strong>activatedby the UV radiation produced by the UV lamp thatpenetrates the microorganism’s cell wall and membrane,destroy<strong>in</strong>g the <strong>in</strong>ner material. These microorganismsrequire a precise wavelength and duration<strong>of</strong> irradiation to be destroyed; otherwise, they canresist, repair, and regrow (M<strong>of</strong>idi et al., 2002). UVsterilisation is a po<strong>in</strong>t-contact process. Efficiencydepends on the time <strong>of</strong> contact; therefore, the speed<strong>of</strong> the flow is crucial. UV sterilisation efficiency isimpeded by turbidity; the higher the number <strong>of</strong> suspendedparticles <strong>in</strong> the water, the less light penetration(Spotte, 1991). The precise UV dosage will bedeterm<strong>in</strong>ed by the quality <strong>of</strong> water and the type <strong>of</strong>microorganisms targeted. UV dose is recorded as aproduct <strong>of</strong> irradiance (milliwatts per square centimetre)and time (second), which provides units <strong>of</strong>milliJoules per square centimetre (mJ/cm 2 ) (M<strong>of</strong>idiet al., 2002). Low-pressure lamps are preferred asthey have a longer life and are more economical toreplace. Recommended dosage is between 20 and35 mJ/cm 2 (calculated at the end <strong>of</strong> lamp life) ata wave length <strong>of</strong> 254 nanometer (nm). UV lightsterilisation is not the most efficient system forlarge volumes (Spotte, 1991); it is also one <strong>of</strong> theless economical (Boness, 1996); however, it issafer <strong>in</strong> comparison with other means <strong>of</strong> sterilisation<strong>in</strong> terms <strong>of</strong> potential production <strong>of</strong> harmfulbyproducts.I recommend that a newly <strong>in</strong>stalled systems berun for several days to several weeks before <strong>in</strong>troduc<strong>in</strong>ganimals <strong>in</strong>to the pool to ensure that thereare no toxicity concerns, leakage, or loose materialsfrom the LSS.In conclusion, there is not one solution that hasall the advantages. The survey showed that newLSSs tend to be more complex and that a comb<strong>in</strong>ation<strong>of</strong> techniques is the most efficient solution.Nowadays, it is common to see an LSS equippedwith high-pressure sand filters as the basicmechanical <strong>in</strong>stallation. This is used <strong>in</strong> comb<strong>in</strong>ationwith either a high dosage <strong>of</strong> ozone or prote<strong>in</strong>skimmers, comb<strong>in</strong>ed with a lower dosage <strong>of</strong>ozone. This also is associated with activated carbonfilters or denitrification filters on side-streams,sometimes a side-stream treated by UV, and <strong>of</strong>tena small dosage <strong>of</strong> chlor<strong>in</strong>e as a residual oxidant <strong>in</strong>bulk-fluid to prolong oxidation capacity and controlalgal growth. This comb<strong>in</strong>ation <strong>of</strong> techniquesallows all <strong>of</strong> the different and extremely complexreactions occurr<strong>in</strong>g <strong>in</strong> cetacean pool water purificationto be tackled with more subtlety.Water Flow and Dra<strong>in</strong>ageFlow mechanics <strong>in</strong> pools are a delicate and complicatedmatter, and they are difficult to summarise.Figure 6.8. UV unit (Photograph from I. Smit)Water circulation depends ma<strong>in</strong>ly on the volume <strong>of</strong>water and the topography and depth <strong>of</strong> the pool. Ingeneral, water flows <strong>in</strong>to the pool through several<strong>in</strong>let pipes located <strong>in</strong> the upper section, and sumpslocated at the bottom <strong>of</strong> the pool collect water tooutlet pipes. Both <strong>in</strong>let and outlet pipes shouldbe placed <strong>in</strong> such a way that they leave no “deadcorner” <strong>in</strong> the pool. The survey <strong>in</strong>dicated that itis sometimes obvious that such pockets <strong>of</strong> poorlyagitated water exist when leaves, food debris, anddirt regularly accumulate at the bottom.Water <strong>in</strong>let pipes also can be located all aroundthe perimeter <strong>of</strong> the pool, close to the water surface.This system is found <strong>in</strong> semi-natural poolswhere pipes can be hidden beh<strong>in</strong>d rocks and aredesigned to distribute water evenly.Air entrapment <strong>in</strong> the water column is acommon problem <strong>in</strong> a water circulation system.High turbidity levels <strong>in</strong>crease air entrapment assuspended solids b<strong>in</strong>d with air bubbles and preventthem from ascend<strong>in</strong>g to the surface and escap<strong>in</strong>g.It is caused by a high water flow rate that producesturbulence <strong>in</strong> some parts <strong>of</strong> the circulation system.It does not cause an immediate health threat to theanimals, but it can severely disrupt their echolocationabilities (Fasik, 1991).Water turnover rate depends on various factorssuch as the volume <strong>of</strong> water to treat or replace,the number <strong>of</strong> animals, the water temperature, andthe sterilisation system. The survey <strong>in</strong>dicated that
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