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BD Biosciences - Discovery Labware Quality control: • Tested for ability to promote mouse splenocyte or human PBMC proliferation • Tested and found negative for presence of bacteria and fungi storage and stability: Stable for at least three months at 2-8°C. Do not freeze. Anti-Mouse CD3 Plates 48-hour incubation with mouse splenocytes on anti-mouse CD3 plates followed by a four hour MTS assay. Anti-Human CD3 Plates 48-hour incubation with human PBMCs followed by cell proliferation assay using BrdU (six-hour labeling). tIp BD Biocoat cell environments Description Plate type Qty. cat. no. assay Plates, with lid Anti-Mouse CD3 96-well, clear 5 354720 Anti-Human CD3 96-well, clear 5 354725 Uncoated Control Plate 96-well, clear 5 354730 Inquire more information about related products from your BD Biosciences representative or contact your local BD office . related products Anti-CD28 Hamster IgG (isotype control) Anti-IL-2 IL-2 OptEIA ELISA Set BD RiboQuant Cytokine Multi-Probe Template Set . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . See BD Biosciences Clinical & Research Catalog 6 cellware bdbiosciences.com | 101
6 cellware BD Biocoat cell environments BD Biosciences - Discovery Labware BD Biocoat variety Pack cellware • Provides convenient and economical substrates for determining the appropriate extracellular matrix for specific cell types and applications BD BioCoat Variety Packs provide several evaluation coatings of different extracellular matrix proteins and attachment factors in order to optimize specific cell cultivation condition. In the above shown example fetal bovine heart endothelial cells (FBHEC) formed after five days a confluent monolayer on Collagen I coated cellware, while still exhibiting numerious mitotic cells. BD Biocoat variety Pack cellware applications include: • Determination of optimal substrate for growth or differentiation of particular cell types • Studies of effects of various ECM components on cell behavior • Cell adhesion assays Quality control: • Tested for ability to promote cell growth or differentiation (cell type used is indicated for each individual BD BioCoat Cellware product) • Tested and found negative for bacteria and fungi storage and stability: Stable for at least three months at 2-8°C. Do not freeze. 102 | bdbiosciences.com Available configurations are either coated CultureSlides for optimized microscopic applications (above) or as a selection of 6-well Multiwell Plates (not shown). Description Qty. cat. no. multiwell Plates variety Pack i includes 6-well plates of: 354417 Collagen I 1 Fibronectin 1 Laminin 1 Poly-D-Lysine 1 BD Falcon uncoated Control 1 variety Pack ii includes 6-well plates of: 354431 Collagen I 1 Collagen IV 1 Fibronectin 1 Laminin 1 Poly-D-Lysine 1 cultureslides includes three cultureslides each of: Collagen I, Fibronectin, Poly-D-Lysine, and BD Falcon uncoated Control 2-well 12 354655 8-well 12 354656
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204 Bioimaging SyStemS BD Bioscienc
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9 Pathway Bioimager Bioimaging SySt
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9 aCtone gPCR Screening assay Bioim
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10 Alphabetical Index AlphAbetIcAl
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10 numerical Index nUMeRIcAl Index
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BD Biosciences Worldwide Discovery
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