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extracellular matrices
BD Biocoat cell environments BD Biosciences - Discovery Labware
vitronectin
Vitronectin, also known as S-protein, serum spreading factor and
epibolin, is a mixture of two monomeric glycoproteins (65 and 75
kD) present in blood and the extracellular matrix (ECM) of many
tissues. Vitronectin and fibronectin are the two major adhesive
proteins in plasma and serum. Like many other adhesion molecules,
vitronectin binds to cells through an interation of the Arg-Gly-Asp
(RGD) sequence in its cell binding domain with vitronectin-specific
cell surface receptors, such as integrins α v ß 3 and α v ß 5 .
Interactions with ECM components are mediated principally
through the collagen-binding domain of vitronectin. By binding to
plasminogen activator inhibitor type I, matrix-bound vitronectin plays
a role in regulating pericellular proteolysis. When used as a thin
coating on tissue culture surfaces, vitronectin is useful to promote
cell attachment, spreading, proliferation, and differentiation of many
normal and neoplastic cells, and to study cell migration.
Description Qty. cat. no.
Vitronectin, human 0 .25 mg 354238
Formulation:
Lyophilized (dialyzed against 10 mM phosphate buffer pH 7.7);
reconstitute in distilled water or buffered solution at neutral pH
source:
Human plasma
note: Source material tested for hepatitis B antigen and HIV-1
antibody
molecular Weight:
75 kD and 65 kD components
Quality control:
• Purity > 95% by SDS-PAGE
• Tested for ability to promote attachment and spreading
of HT-1080 human fibrosarcoma cells
• Filtered (0.2 µm membrane), tested, and found negative for
bacteria, fungi, and mycoplasma
instructions for Use:
Recommended concentration — 50 ng/cm 2 of growth surface
depending on cell type. Guidelines for coating included with product.
storage and stability:
Stable for at least three months at 2-8°C (lyophilized) or one week
at 2-8°C (solubilized). Do not store in frost-free freezer. avoid
multiple freeze-thaws.
136 | bdbiosciences.com