Tubes - BD

BD Biosciences - Discovery Labware
source:
Engelbreth-Holm-Swarm (EHS) mouse tumor
Formulation:
Dulbecco’s Modified Eagles’ Medium with 50 µg/ml gentamycin.
BD Matrigel Matrix is compatible with all culture media.
Quality control:
• BD BioCoat Matrigel Matrix Cellware is tested for ability
to promote neurite outgrowth from chick dorsal root
ganglia (in the absence of NGF)
• Tested and found negative for bacteria and fungi
storage and stability:
BD BioCoat Matrigel Matrix Cellware is stable for at least three
months at –20°C. Keep frozen until use. BD BioCoat Matrigel
Matrix Cellware, thin layer is stable for at least three months at
2-8°C.
REFERENCES:
1. Vukicevic, S., et al., Exp. Cell Res. 202:1 (1992).
2. Yeh, C-K., et al., In Vitro Cell. Dev. Biol. 27a:707 (1991).
3. Hartley, R.S. and Yablonka-Reuveni, R., In Vitro Cell. Dev. Biol. 26:955 (1990).
4. Dym, M., et al., Endocrin. 128(2):1167 (1991).
5. Grant, D.S., et al., In Vitro Cell. Dev. Biol. 27a:327 (1991).
6. Barcellos-Hoff, M.H., et al., Dev. 105:223 (1989).
7. Boyce, J.A., et al., J. Exp. Med. 182:49 (1995).
8. Shakado, S., et al., Hepatology 22(3):969 (1995).
9. Yoshino, K., et al., Exp. Cell Res. 220:138 (1995).
10. Sullivan, D.A., et al., J. Immun. 145(12):4238 (1990).
11. Doron, D.A., et al., In Vitro Cell. Dev. Biol. 27a:689 (1991).
12. Funanage, V.L., et al., J. Cell. Physiol. 150:251 (1992).
13. Mathis, G.A. and Siric, A.E., In Vitro Cell. Dev. Biol. 26:113 (1990).
14. Smith, S.M., BioTechniques 11(3):7 (1991).
15. Arslan, A., et al., In Vitro Cell. Dev. Biol. 31:140 (1995).
16. Younkin, D.P., et al., Proc Natl. Acad. Sci. USA 90:2174 (1993).
17. Absher, M. and Baldor, L., In Vitro Cell. Dev. Biol. 27a:25 (1991).
18. Diamond, L.E., et al., Transplantation 61(8):1241 (1996).
19. Munro, C., et al., J. Biol. Chem. 280(2):1051 (2005).
20. Fabrega, A., et al., Transplantation 62(12):1866 (1996).
Further references for BD Matrigel Matrix are listed on page 125.
tIps
SPECIAL HANDLING: Store
BD BioCoat Matrigel Matrix
Cellware at –20°C, thaw at 4°C
overnight . Do not thaw in a
warm waterbath . Plates must
be thawed on a level surface
in order for the BD BioCoat
Matrigel Matrix to be evenly
distributed . To form a stable
gel, place the thawed plate at
35-37°C for 30 minutes . Do not
refreeze plates .
Recover cells cultured on
BD BioCoat Matrigel Matrix with
BD Dispase (Cat . No . 354235) or
BD Cell Recovery Solution (Cat . No .
354253) .
BD Biocoat cell environments
BD Biocoat matrigel matrix cellware
BD BioCoat Matrigel Matrix Cellware is a line of tissue culture vessels
with a uniform application of BD Matrigel Matrix. This cellware is
manufactured in a highly controlled environment and rigorously
tested to assure product consistency and performance.
Description Qty. cat. no.
BD Biocoat matrigel matrix multiwell Plates
6-well 2 354432
12-well 2 354503
24-well 2 354433
48-well 2 354508
BD Biocoat matrigel matrix culture Dishes
35 mm 8 354460
BD Biocoat matrigel matrix thin-layer multiwell
and assay Plates
6-well 5 354603
24-well 5 354605
96-well 5 354607
BD Biocoat matrigel matrix thin-layer culture
Dishes
35 mm 20 354602
60 mm 20 354601
100 mm 10 354600
BD Biocoat matrigel matrix for Hepatocytes
6-well plates 5 354510
100 mm culture dishes 5 354634
related products
BD BioCoat Matrigel
Matrix Cell Culture Inserts . . . . . . . . . . . . . 111
Vialed BD Matrigel Matrices . . . . . 124
BD BioCoat Matrigel
Invasion Chambers . . . . . . . . . . . . . . . . . . . . . . . 74
BD BioCoat Angiogenesis
Products . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 148
BD Dispase . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 126
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cellware
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