Tubes - BD

6
scaffolds
BD Biocoat cell environments BD Biosciences - Discovery Labware
BD three Dimensional collagen composite scaffolds
• Support short- and long-term cell growth and differentiation
• Suitable for in vitro and in vivo basic research
• Highly biocompatible and bioresorbable natural material
One of the most common biomaterials used for three dimensional
(3D) cultivation is collagen. The major advantage of natural collagens
is that they are highly biocompatible, biodegradable, and safe.
Their abundance, the ability to support the growth of a wide variety
of tissues and a relative lack of immunogenicity are additional
positive aspects. Depending on the experimental design, polymers
of collagen can either be formed as 3D gel 1,2 or as 3D structure of
a fibrillar network 3 . It has also been tried to coat pieces of sponges
with collagen 4 .
The BD 3D Collagen Composite Scaffolds are manufactured from
a proprietary mixture of collagens that are derived from bovine
hide. This material exhibits collagen fibrillar architecture, which
is representative of the natural structure of collagen within the
interstitial matrix. These ready-to-use scaffolds are a convenient
substrate in 3D culture applications.
118 | bdbiosciences.com
Scanning electron microscope image of a
collagen composite scaffold.
BD 3D collagen composite scaffold
applications include:
• Promotion of cell growth and differentiation (e.g.,
MC3T3-E1 osteoblasts, WI-38 human lung fibroblasts,
primary rat hepatocytes)
• ECM pre-incubation for specific stem/progenitor cell
differentiation assays
• Dynamic cell seeding in larger vessels (e.g., BD Falcon
50 ml Conical Tubes)
• Non-invasive evaluation of cell growth using the BD Oxygen
Biosensor System