Tubes - BD

6
extracellular matrices
BD Biocoat cell environments BD Biosciences - Discovery Labware
BD matrigel Basement membrane matrix
• Promotes differentiation of many cell types
• Gels to form a three-dimensional model of basement
membrane
• Increases rate of human tumor cell growth in nude mice
• Creates models to measure invasive potential of tumor cells
BD Matrigel Basement Membrane Matrix is a solubilized basement
membrane preparation extracted from the Engelbreth-Holm-Swarm
(EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins.
Its major component is laminin, followed by collagen IV, entactin, and
heparan sulfate proteoglycan 1 . It also contains TGF-ß fibroblast growth
factor, tissue plasminogen activator 2 , and other growth factors that
occur naturally in the EHS tumor.
At room temperature, BD Matrigel Matrix polymerizes to produce a
biologically active matrix material resembling the mammalian cellular
basement membrane.
amounts of Growth Factors (GF) Present in
BD matrigel matrix vs. GFr BD matrigel matrix
124 | bdbiosciences.com
range of GF average GF Typical GF
Growth Concentration in Concentration in Concentration in GFr
Factor BD Matrigel Matrix BD Matrigel Matrix BD Matrigel Matrix
EGF 0 .5-1 .3 ng/ml 0 .7 ng/ml < 0 .5 ng/ml
bFGF < 0 .1-0 .2 pg/ml n .a .* n .d .**
NGF < 0 .2 ng/ml n .a .* < 0 .2 ng/ml
PDGF 5-48 pg/ml 12 pg/ml < 5 pg/ml
IGF-1 11-24 ng/ml 16 ng/ml 5 ng/ml
TGF-ß 1 .7-4 .7 ng/ml 2 .3 ng/ml 1 .7 ng/ml
* n.a. - not applicable
** n.d. - not determined
Human Submandibular Gland (HSG)
cells cultured on GFR BD Matrigel Matrix
differentiate to form acinar structures
within 24 hours. The acini shown above
were stained with H/E at 72 hours.
Indirect immunofluorescence staining
also revealed the salivary gland specific
cysteine protease inhibitor, cystatin, in
HSG cell acini (data not shown). (Photo
courtesy of Dr. Hynda Kleinman).
BD Matrigel Basement Membrane Matrix is effective for the
attachment and differentiation of both normal and transformed,
anchorage-dependent epithelial and other cell types. These include
neurons, hepatocytes, Sertoli cells, mammary epithelial, melanoma
cells, vascular endothelial cells, thyroid cells, and hair folicle cells 3-6 .
BD Matrigel Basement Membrane Matrix will influence casein gene
expression in mouse mammary epithelial cells 7-8 . It will also support
in vivo peripheral nerve regeneration 9 and facilitates differentiation of
bovine oviduct epithelial cells 10 .
The Growth Factor Reduced (GFR) product is useful as an alternative
to BD Matrigel Matrix in many of the applications listed above where
a more highly defined basement preparation is desired. It has been
used to define the signals necessary in the formation of canicular cell
processes in bone cells 11 , in elucidating the role of growth factors
in the formation of tubules by primary mouse kidney cells 12 , and in
gene expression studies of primary mouse mammary epithelial cells 13 .
The phenol red-free product is suitable for those assays that require
color detection (i.e. fluorescence).
All BD Matrigel Basement Membrane Matrices including the High
Concentration formulation can also be used to assess in vivo
angiogenic activity of different compounds by subcutaneous injection
into mice (BD Matrigel Plug Assay) 14-18 . A similar approach can be
used for the transplantation and investigation of human tumor
cells. Reports include studies with prostatic, breast, small-cell lung,
colon, adrenal carcinomas, melanomas, and lymphoblastic leukemia
cells 19-24 .
ecm composition of BD matrigel matrix
vs. GFr BD matrigel matrix GFr
Basement Percent in Percent in
membrane BD Matrigel GFr BD Matrigel
matrigel Matrix Matrix
component
Laminin 56% 61%
Collagen IV 31% 30%
Entactin 8% 7%