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laminin
Laminin, a major component of basement membranes, has numerous
biological activities including promotion of cell adhesion, migration,
growth, and differentiation, including neurite outgrowth. It can
be used as a thin coating on tissue culture surfaces or as a soluble
additive to culture medium. Laminin has been shown in culture
to stimulate neurite outgrowth, and promote cell attachment,
chemotaxis, and cell differentiation.
The Laminin/Entactin Complex High Concentration (HC) is a special
formulation that has been developed for three dimensional (3D)
culture. Culturing cells in or on gels such as the Laminin/Entactin
Complex will enable the study of specific mechanisms that dictate cell
differentiation and functionality. Application examples are endothelial
cell tubulogenesis 1 and acinar differentiation 2,3 .
See page 90 for more information on Laminin.
NG-108 neuroblastoma cells cultured on BD BioCoat Laminin Cellware exhibit a spindleshaped
morphology and dendritic processes.
Description Qty. cat. no.
BD Laminin, mouse 1 mg 354232
Description Qty. cat. no.
BD Laminin/Entactin Complex
High Concentration, mouse 10 .5 mg 354259
REFERENCES:
1. Grant, D.S., et al., Cell, 58:933 (1989).
2. Hoffman, M.P., et al., J. Biol. Chem., 273:28633 (1998).
3. Zheng, C., et al., J. Cell. Physiol., 177:628 (1998).
BD Biocoat cell environments
Formulation:
Standard: 1 mg/ml frozen in 0.05 M Tris-HCl, 0.15 M NaCl, pH 7.4
HC Laminin/Entactin Complex: ≥ 10 mg/ml frozen in Dulbecco´s
Phosphate-Buffered Saline
source:
Engelbreth-Holm-Swarm mouse tumor
molecular Weight:
900 kD (unreduced)
Quality control:
• Purity > 90% by SDS-PAGE (contains entactin)
• Tested for ability to initiate differentiation (neurite outgrowth) of
NG-108 rat glioma/mouse neuroblastoma cells
• Filtered (0.2 µm membrane), tested, and found negative for
bacteria, fungi, and mycoplasma
instructions for Use:
Recommended concentration — 1-10 µg/cm 2 of growth surface
depending on cell type. HC Laminin/Entactin Complex provides a
3D matrix and cell can either be cultured on top or within the gel.
Guidelines included with product.
storage and stability:
Stable for at least three months at -70°C. Do not store in frost-free
freezer. avoid multiple freeze-thaws.
Description Qty. cat. no.
BD Ultrapure Laminin, mouse
(entactin-free) 1 mg 354239
Formulation:
Frozen in 0.05 M Tris-HCl, 0.15 M NaCl, pH 7.4
source:
Engelbreth-Holm-Swarm mouse tumor
molecular Weight:
900 kD (unreduced)
Quality control:
• Purity > 95% by SDS-PAGE. (Highly purified laminin preparation
that is free of entactin. Entactin is a 150 kD protein tightly
bound to laminin, serving as a bridge between laminin and
collagen IV.)
• Tested for ability to initiate differentiation (neurite outgrowth) of
NG-108 rat glioma/mouse neuroblastoma cells
• Filtered (0.2 µm membrane), tested, and found negative for
bacteria, fungi, and mycoplasma
instructions for Use:
Recommended concentration — 1-10 µg/cm 2 of growth surface
depending on cell type. Guidelines for coating included with product.
storage and stability:
Stable for at least three months at -70°C. Do not store in frost-free
freezer. avoid multiple freeze-thaws.
related products
BD BioCoat Laminin coated
Cellware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
BD BioCoat Laminin coated
Cell Culture Inserts . . . . . . . . . . . . . . . . . . . . . 111
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extracellular matrices
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