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BD Biosciences - Discovery Labware BD Biocoat Poly-lysine cellware BD Biocoat cell environments BD BioCoat Poly-Lysine Cellware is a line of tissue culture vessels with a uniform application of poly-lysine. This cellware is manufactured in a highly controlled environment and vigorously tested to assure product consistency and performance. Description Plate Qty. cat. no. Qty. cat. no. Qty. cat. no. type BD Biocoat Poly-D-lysine cellware multiwell and assay Plates 6-well 5 354413 50 356413 12-well 5 354470 50 356470 24-well 5 354414 50 356414 48-well 5 354509 50 356509 96-well Clear 5 354461 50 356461 80 356690 96-well Black/Clear 5 354640 50 356640 80 356692 96-well White/Clear 5 354651 50 356651 80 356693 96-well White 5 354620 50 356620 80 356691 384-well Black/Clear 5 354663 50 356663 80 356697 384-well White/Clear 5 354660 50 356660 80 356694††† 384-well Clear 5 354662 50 356662 80 356696††† 384-well White 5 354661 50 356661 80 356695††† culture Dishes 35 mm 20 354467 100 356467 60 mm 20 354468 100 356468 100 mm 10 354469 40 356469 150 mm 5 354550 – – Flasks 25 cm 2 plug-seal † 10 354479 50 356479 25 cm 2 vented-cap † 10 354536 50 356536 75 cm 2 plug-seal †† 5 354524 50 356524 75 cm 2 vented-cap †† 5 354537 50 356537 150 cm 2 plug-seal 5 354495 40 356495 150 cm 2 vented-cap 5 354538 40 356538 175 cm 2 plug-seal 5 354529 40 356529 175 cm 2 vented-cap 5 354539 40 356539 coverslips (no. 1 German Glass) 12 mm Round 80 354086 coverslip-Bottom Dishes (no. 1 German Glass) 35 mm dish with coverslip bottom 20 354077 cultureslides 1-well – – 12 354566 2-well – – 12 354629 4-well – – 12 354577 8-well – – 12 354632 † BD BioCoat T-25 Flasks are 70 ml canted neck †† BD BioCoat T-75 Flasks are 250 ml canted neck ††† Please call for shipping schedules on large orders Description Plate Qty. cat. no. Qty. cat. no. type BD Biocoat Poly-l-lysine cellware multiwell and assay Plates 6-well 5 354515 50 356515 96-well Clear 5 354516 50 356516 culture Dishes 35 mm 20 354518 100 356518 60 mm 20 354517 100 356517 coverslips (no. 1 German Glass) 12 mm Round 80 354085 REFERENCES: 1. McKeehan, W.L., Methods for Preparation of Media, Supplements and Substata for Serum-free Animal Cell Culture, A.R. Liss, NY, p.209 (1984). 2. LaRocca, P. and Barker, S., The Cell/Line 6(2):1 (1996). 3. Yavin, E. and Yavin, Z., J. Cell Biol. 62:540 (1974). 4. Letourneau, P.C., Dev. Biol. 44-77 (1975). 5. McKeehan, W.L. and Ham, R.G., J. Cell. Biol. 71:727 (1976). 6. Qian, Y., et al., J. Neurosci. 17(1):45 (1997). 7. Alvarez, C.V., et al., J. Biol. Chem. 270 (27):16271 (1995). 8. Thomas, D., et al., J. Biol. Chem. 270(48):28924 (1995). 9. Nitsch, R.M., et al., J. Biol. Chem. 271(8):4188 (1996). 10. Srinivasan, A., et al., J. Neurosci. 16(18):5654 (1996). 11. Parks, C.L. and Shenk, T., J. Biol. Chem. 271(8):4417 (1996). 12. Courtney, M.J., et al., J. Biol. Chem. 270(36):21271 (1995). 13. Vincent, B., et al., J. Neurosci. 16(16):5049 (1996) 14. Craig, A.M., et al., Neuron 10:1055 (1993). 15. Hori, O., et al., J. Biol. Chem. 270(43):25752 (1995). 16. Lee, M.K., et al., J. Neurosci. 16(23):7513 (1996). 17. Nonner, D., et al., J. Neurosci. 16(21):665 (1996). 18. Cohen, R.I., et al., J. Neurosci. 16(20):6433 (1996). 19. Tocher, D., J. Methods Neurosci. 33:993 (1990). 20. Komiyana, A., Dev. Brain Res. 62:7 (1991). 21. Benjamin, E., et al., J. Biomol Screening 9(4):343 (2004). 22. Hu, F., et al., J. Neuroscience 25(22):5298 (2005). 23. Mizrachi, D and Segaloff D.L., Molecular Endrocrinology 18(7):1768 (2004). 24. Kamiya, K., et al., J. Neuroscience 24(11):2690 (2004). 25. Tao, Y.X., et al., J. Biol. Chem. 279(7):5904 (2004) 26. Malagelada, C., et al., Stroke 35:2396 (2004). 27. Perry, S.J., et al., J. Biol. Chem. 280(12):11560 (2005). 28. Silverman, E., et al., Molecular and Cellular Biology 24(24):10573 (2004). 29. Mukhopadhyay, S., et al., Nucleic Acids Research 32(19):5820 (2004). 30. Kushnareva Y.E., et al., J. Biol. Chem. 280(32):28894 (2005). 31. Hillion, J.A., et al., J. Cerebral Blood Flow & Metabolism 25:154 (2005). related products BD BioCoat Variety Pack Cellware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103 BD BioCoat Poly-D-Lysine Mixtured Cellware . . . . . . . . . . . . . . . . . . . . . . . . . . 98 BD BioCoat Poly-Lysine in Drug Discovery & Dev . . . . . . . . . . . . . . . . 164 Vialed BD Poly-D-Lysine . . . . . . . . . . . . . . . . 134 6 cellware bdbiosciences.com | 97
6 cellware BD Biocoat cell environments BD Biosciences - Discovery Labware BD Biocoat Poly-D-lysine/laminin, Poly-l-ornithine/laminin, and laminin/Fibronectin cellware • Promotes neuronal cell attachment and differentiation for a variety of applications RCG cells cultured on BD BioCoat PLO/LM Cellware exhibit a spindle-shaped morphology and dendritic processes. BD BioCoat Poly-D-Lysine/Laminin (PDL/LM) and Poly-L-Ornithine/ Laminin (PLO/LM) Cellware is suitable for culturing many different types of peripheral and central nervous system (CNS) networks and is useful for promoting neural cell attachment and differentiation. BD BioCoat Laminin/Fibronectin (LM/HFN) Cellware provides an in vitro environment that promotes cell attachment and extensive process formation. In addition, this substrate upregulates receptor systems that are integral to neuronal communications. BD BioCoat LM/HFN Cellware facilitates the study of pharmacological or physiological events of the CNS by providing molecular cues required to produce neuronal cultures capable of responding to many important neurotransmitters, neuropeptides, and excitatory amino acids such as glutamate, NMDA, and kainate 1 . BD Biocoat PDl/lm, Plo/lm and lm/HFn cellware applications include: • Enhancement of neuronal cell attachment to plastic and glass • Promotion of neurite outgrowth • Culture of glial cells as feeder layers for neurons • Construction of neural cell model systems to study CNS function development and diseases 98 | bdbiosciences.com Neurons plated on BD BioCoat LM/HFN Cellware form extensive connections. Once adherence to the substrate occurs, there is extension of the growth cone as it grows toward a target. Neurites respond to neurotransmitter by extending filopodia which contain actin. the following cell types have been cultured on BD Biocoat PDl/lm or Plo/lm cultureware: • Embyronic rat sympathetic neurons 2 • Glial feeder layers as a substrate for neurons 3 • Embyronic dorsal root ganglia (DRG) 4 • E18 hippocampal neurons 5 • Mouse striatal cells 6 • Cerebellar macroneurons 7 • Rat astrocytes 8 • Adult human bone marrow stem cells 11 • N2a, Sc2Na neuroral cells 9 • Primary embryonic cortical neurons 10
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204 Bioimaging SyStemS BD Bioscienc
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9 Pathway Bioimager Bioimaging SySt
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9 aCtone gPCR Screening assay Bioim
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10 Alphabetical Index AlphAbetIcAl
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10 numerical Index nUMeRIcAl Index
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BD Biosciences Worldwide Discovery
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