Tubes - BD

More documents

Recommendations

Info

BD Biosciences - Discovery Labware Supplement BD ACTOne GPCR Screening Assay • Convenient, homogeneous assay format • High sensitivity and excellent signal-to-noise ratio • Live cell assay allows simultaneous screening of agonists and antagonists • No special equipment required • Z’ scores are consistently greater than 0.6 Diagram shows two pathways of GPCR signalling. The BD ACTOne Assay uses a proprietary method cyclic nucleotide gated channel (CNG) as a biosensor of cAMP activity. The BD ACTOne live cell cAMP assay is the only high-throughput screening (HTS) G-protein coupled receptor (GPCR) technology that directly measures the intracellular concentration of the secondary messenger cyclic AMP (cAMP) in living cells, in real-time. Because the biosensor is localized at the cell surface near the source of cAMP production, the assay provides exceptional sensitivity and response time. Description Cat. No. Transduced Gs coupled Receptors Adenosin A2a Receptor 341449 Adenylate Cyclase Activ. Polypept. 1 Receptor Type I (ADCY AP1R1) 341815 Arginine Vasopressin Receptor 2 (AVPR2) 345502 Calcitonin Receptor (CALCR) 341452 Calcitonin receptor-like (CALCRL) 341450 Corticotropin Releasing Hormone Receptor 1 (CRHR1) 345505 Corticotropin Releasing Hormone Receptor 2 (CRHR2) 341816 Dopamine Receptor D1 (DRD1) 341459 Dopamine Receptor D5 (DRD5) 341817 Follicle Stimulating Hormone Receptor (FSHR) 341818 Glucagon Receptor (GCGR) 341471 Glucagon-like peptide 1 Receptor (GLP1R) 345504 Glucagon-like peptide 2 Receptor (GLP2R) 344445 Gastric inhibitor polypeptide Receptor (GIPR) 341473 Melanocortin 1 Receptor (MC1R) 341457 Melanocortin 3 Receptor (MC3R) 344447 Melanocortin 4 Receptor (MC4R) 341469 Melanocortin 5 Receptor (MC5R) 341470 Parathyroid Hormone Receptor 1 (PTHR1) 341442 Parathyroid Hormone Receptor 2 (PTHR2) 341820 Prostaglandin D2 Receptor (PTGDR2) 341443 Prostaglandin E Receptor 4 (PTGER4) 341455 Prostaglandin I2 Receptor (PTGIR) 345503 Secretin Receptor (SCTR) 344439 Serotonin Receptor 4 (5HT4) 344438 Serotonin Receptor 6 (5HT6) 344442 Serotonin Receptor 7 (5HT7) 344444 Thyroid Stimulating Hormone Receptor (TSHR) 344446 Vasoactive Intestinal Peptide Receptor 1 (VIPR1) 341472 Vasoactive Intestinal Peptide Receptor 2 (VIPR2) 341468 Live cell assays BIOIMAGING SYSTEMS Traditional screening of Gs- and Gi-coupled GPCRs is based on endpoint assays that do not generate real-time kinetic data about Gs- and Gi-mediated cAMP activation. The cAMP assay from BD Biosciences allows high-throughput screening on industry standard platforms, providing results with greater accuracy at lower cost. The BD ACTOne assay is also compatible with standard fluorescence plate readers. The figure shows how the assay uses a modified cyclic nucleotide gated channel (CNG) to monitor cAMP levels within cells. The BD ACTOne assay represents a major breakthrough in the ability to conduct HTS using fluorescence plate readers instead of slower, more costly alternatives. Real-time kinetic readouts minimize artifacts from prolonged incubations and decrease processing steps while providing higher statistical relevance. Kinetic traces also allow easy differentation between GPCR response patterns, making the BD ACTOne assay ideal for receptor deorphanization. BD ACTOne Cell Lines A patented CNG mutant gene was stably introduced into HEK-293 cells as a biosensor for intracellular cAMP in the presence of selected fluorescent dyes. The BD ACTOne Cell Lines can be used to detect and quantitate free intracellular cAMP in real time, providing a kinetic or endpoint assay for both endogenous and exogenous GPCRs. BD Biosciences also offers a specially formulated fluorescent membrane potential dye that has been pharmacologically selected to minimize binding to a peptide or protein agonist. The dye can be used with the BD ACTOne Assays or with other fluorescence-based membrane potential assays. Cell lines are available under an evaluation or screening license agreement. Description Cat. No. Endogenous Gs-coupled Receptors β-adrenoreceptor (likely both β 1 & β 2) 341475 Adenosine A2b Receptor 341474 Prostaglandin E2 Receptor 341476 Adenosine A1 Receptor 341814 Adenosine A3 Receptor 341822 NEW Angiotensin receptor-like 1 644564 Cannabinoid Receptor 1 (CB1) 341811 Cannabinoid Receptor 2 (CB2) 344449 Chemokine (C-C motif) receptor 5 (CCR5) 344448 NEW Chemokine (C-X-C motif) receptor 4 (CXCR 4) 644562 Dopamine Receptor D2 (DRD2) 341812 NEW Glutamate Receptor, metabotropic 2 (mGluR2) 644561 NEW Glutamate Receptor, metabotropic 4 (mGluR4) 644565 NEW Glutamate Receptor, metabotropic 7 (mGluR7) 644560 Glutamate Receptor, metabotropic 8 (mGluR8) 344451 Melanin concentrating hormone Receptor 1 (MCHR1) 341823 Neuropeptide Y Receptor Y1 (NPY1R) 344869 Neuropeptide Y Receptor Y2 (NPY2R) 344870 NEW Neuropeptide Y Receptor Y4 (NPY4R) (PPYR1) 644009 Opioid Receptor, b β1 (KOR) 344450 NEW Opiate Receptor-like Receptor 1 (ORL-1) 644563 Somatostatin Receptor 5 (SSTR5) 341813 NEW Sphingolipid GPCR 1 640236 Parental Cell Lines Parental Cell line HEK-293 CNG 341467 GLAST (transfected with the GLAST gene, a glutamate/ aspartate transporter) * 344443 BD ACTOne Membrane Potential Dye Kits 10 plates 341831 100 plates 341833 * this cell line should be used as the parental control cell line for mGluR7 and mGluR8 cell lines bdbiosciences.com 33 9 BD Calcium Assay Kits
9 BD Calcium Assay Kits 34 BIOIMAGING SYSTEMS Page 209 of main catalog. BD Calcium Assay Kits • No-wash homogeneous assay format • Higher signal over background compared to competitive kits • PBX kit permits assays with reduced or no probenecid • Ideal for all fluorescence-based calcium flux assays A B BD Calcium Dye Kits have been optimized for detecting calcium flux in living cells. They provide increased signal-to-background in an easy-to-use no wash assay format. In most cases, the dye kits perform better than kits currently available on the market with no known interference to ligands (small molecules, peptide/proteins and lipids). Also, the “addition artifact” commonly seen in this application is significantly reduced with the new dye formulations. The BD kits can be used on different fluorescence-based readers for compound screening. In particular, the PBX-format is suitable for the BD ACTOne GPCR screening technology. Some of the cell lines successfully used with the BD Calcium Dye Kits include: • CHO cells • HEK-293 cells • Jurkat cells • HeLa cells • U2OS cells bdbiosciences.com BDTM BDTM Calcium Assay Kit BDTM BD PBX TM PBX Calcium Assay Kit Competitor Kit BD Biosciences - Discovery Labware Supplement Competitor Kit BDTM BDTM Calcium Assay Kit BDTM BD PBX TM PBX Calcium Assay Kit Comparison of BD Calcium Assay kits with a competitive product used with CHO and HEK-293 cells in the presence of Probenecid. Both BD kits outperform a competitive product, providing significantly higher signal over background. The example on the right also shows the absence of an “addition artifact,” a disadvantage seen using the competitive product. Description Cat. No. For Standard Applications BD Calcium Assay Kit, volumes sufficient for 10 plates 640176 BD Calcium Assay Kit, volumes sufficient for 100 plates 640178 For Probenicid Sensitive Applications BD PBX Calcium Assay Kit, volumes sufficient for 10 plates 640175 BD PBX Calcium Assay Kit, volumes sufficient for 100 plates 640177 Plate formats can either be 96-, 384-, or 1536-well.
Page 2 and 3:
BD Biosciences - Discovery Labware
Page 4 and 5:
Page 6 and 7:
Page 8 and 9:
Page 10 and 11:
Page 12 and 13:
Page 14 and 15:
Page 16 and 17:
Page 18 and 19:
Page 20 and 21:
Page 22 and 23:
Page 24 and 25:
Page 26 and 27:
Page 28 and 29:
Page 30 and 31:
Page 32 and 33:
Page 34 and 35:
Page 36 and 37:
Page 38 and 39:
Page 40 and 41:
Page 42 and 43:
Page 44 and 45:
Page 46 and 47:
Page 48 and 49:
Page 50 and 51:
Page 52 and 53:
Page 54 and 55:
Page 56 and 57:
Page 58 and 59:
Page 60 and 61:
Page 62 and 63:
Page 64 and 65:
Page 66 and 67:
Page 68 and 69:
Page 70 and 71:
Page 72 and 73:
Page 74 and 75:
Page 76 and 77:
Page 78 and 79:
Page 80 and 81:
Page 82 and 83:
Page 84 and 85:
Page 86 and 87:
Page 88 and 89:
Page 90 and 91:
Page 92 and 93:
Page 94 and 95:
Page 96 and 97:
Page 98 and 99:
Page 100 and 101:
Page 102 and 103:
Page 104 and 105:
Page 106 and 107:
Page 108 and 109:
Page 110 and 111:
Page 112 and 113:
Page 114 and 115:
Page 116 and 117:
Page 118 and 119:
Page 120 and 121:
Page 122 and 123:
Page 124 and 125:
Page 126 and 127:
Page 128 and 129:
Page 130 and 131:
Page 132 and 133:
Page 134 and 135:
Page 136 and 137:
Page 138 and 139:
Page 140 and 141:
Bd Biosciences - Discovery Labware
Page 142 and 143:
Page 144 and 145:
Page 146 and 147:
Page 148 and 149:
Page 150 and 151:
Page 152 and 153:
Page 154 and 155:
Page 156 and 157:
Page 158 and 159:
Page 160 and 161:
Page 162 and 163:
Page 164 and 165:
Page 166 and 167:
Page 168 and 169:
Page 170 and 171:
Page 172 and 173:
Page 174 and 175:
Page 176 and 177:
Page 178 and 179:
Page 180 and 181:
Page 182 and 183:
Page 184 and 185:
Page 186 and 187:
Page 188 and 189:
Page 190 and 191:
Page 192 and 193:
Page 194 and 195:
Page 196 and 197:
Page 198 and 199:
Page 200 and 201:
Page 202 and 203:
Page 204 and 205:
204 Bioimaging SyStemS BD Bioscienc
Page 206 and 207: 9 Pathway Bioimager Bioimaging SySt
Page 208 and 209: 9 aCtone gPCR Screening assay Bioim
Page 210 and 211: 10 Alphabetical Index AlphAbetIcAl
Page 220 and 221: 10 numerical Index nUMeRIcAl Index
Page 222 and 223: 10 numerical Index nUMeRIcAl Index
Page 226 and 227: BD Biosciences - Discovery Labware
Page 274: BD Biosciences Worldwide Discovery
show all

Tubes - BD

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?