Tubes - BD

4
cell cultureware
B D F a l c o n c e l l c u lt u r e w a r e BD Biosciences - Discovery Labware
working Volumes for tissue culture Vessels
Dishes
58 | bdbiosciences.com
Volume of Media Volume of trypsin* actual Growth area
(per item) (per item) (per item)
35 mm x 10 mm Style 2 .5-3 ml 0 .2-0 .3 ml 11 .78 cm 2
60 mm x 15 mm Style 6-7 ml 0 .5-0 .6 ml 21 .29 cm 2
100 mm x 20 mm Style 16-17 .5 ml 1 ml 58 .95 cm 2
150 mm x 25 mm Style 45-50 ml 1 .5 ml 156 .36 cm 2
Multiwell Plates
Flasks
*Your lab protocol may call for another proteolytic enzyme.
Volume of Media Volume of trypsin* actual Growth area
(per item) (per item) (per item)
6-well plate 2 .5-3 ml 0 .2-0 .3 ml 9 .6 cm 2
12-well plate 1 .5-2 .2 ml 0 .1-0 .2 ml 3 .8 cm 2
24-well plate 0 .8-1 ml 0 .08-0 .1 ml 2 cm 2
48-well plate 0 .5-0 .8 ml 0 .05-0 .08 ml 0 .75 cm 2
96-well plate 0 .1-0 .2 ml 0 .01-0 .02 ml 0 .32 cm 2
Volume of Media Volume of trypsin* actual Growth area
(per item) (per item) (per item)
12 .5 cm 2 4-5ml 0 .25-0 .40 ml 12 .5 cm 2
25 cm 2 8-9 ml 0 .50-0 .80 ml 25 cm 2
75 cm 2 20-30 ml 1 ml 75 cm 2
150 cm 2 40-50 ml 2 ml 150 cm 2
175 cm 2 45-55 ml 2 ml 175 cm 2
225 cm 2 60-100 ml 4-5 ml 225 cm 2
300 cm 2 150-400 ml 4 ml 300 cm 2
Roller Bottles
Volume of Media Volume of trypsin* actual Growth area
(per item) (per item) (per item)
Smooth 125-400 ml 10-15 ml 850 cm 2
Pleated 200-400 ml 20 ml 1450 cm 2