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BD Biosciences - Discovery Labware RFU 50000 45000 40000 35000 30000 25000 20000 15000 10000 5000 0 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Time (days) Proliferation of WI-38 Human Lung Fibroblasts cultured on BD 3D Collagen Composite Scaffolds indicated by oxygen consumption. The BD Oxygen Biosensor System was used to analyze the oxygen consumption rate (OCR) over time. A polynomial fit to the OCR points was added. 100000 10000 Primary Rat Hepatocytes on Collagen Scaffold 1000 0 2 4 6 8 10 12 14 time (days) Functional assessment over time of primary rat hepatocytes cultured on the BD 3D Collagen Composite Scaffold. Oxygen consumption rate (OCR, analyzed with the BD Oxygen Biosensor System), cell number (computed from Pico Green fluorescence) and specific CYP3A4 activity (measured with the BD Gentest Cytochrome P450 Inhibition Kit and reported as fluorescence divided by cell number) are given. Compared with results from BD OPLA Scaffolds rat hepatocytes show low rates of proliferation but much higher specific P450 activity on collagen scaffolds. REFERENCES: 1. Douglas, W.H.J., et al., In Vitro 16:306 (1980). 2. Yang, J., et al., Cancer Res. 41:1021 (1981). 3. Swiderek, M., et al., FASEB J. 8:4 (1994). 4. Leighton, J., et al., Cancer Res. 28:286 (1968). 1 0 0 0 Cell # OCR p450 BD Biocoat cell environments characteristics: Scaffold Material: Proprietary mixture of type I and III collagens derived from bovine hide Dimensions (fit into a well of a 96-well plate): • 5 mm (diameter) • 3 mm (height) • 0.039 cm 3 (volume) Hydration Capacity: 25 µl Average Pore Size: 100–200 µm Property: Sponge-like structure/compressible storage and stability: Stable for at least three months from the date of shipping when stored at 4-30°C. Description Qty. cat. no. BD 3D Collagen Composite Scaffolds 24 354613 related products Vialed BD Collagens . . . . . . . . . . . . . . . . . . . 128 BD PuraMatrix Peptide Hydrogel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135 BD Oxygen Biosensor System . . . . 180 BD Gentest Cryopreserved Hepatocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .see BD Gentest Catalog 6 scaffolds bdbiosciences.com | 119
6 scaffolds BD Biocoat cell environments BD Biosciences - Discovery Labware BD three Dimensional oPla ® scaffolds • Support short- and long-term growth and differentiation of a variety of cells • Suitable for in vitro and in vivo basic research • Biocompatible synthetic polymer Besides natural components synthetic polymers are another active field for bioengineered three dimensional (3D) scaffold investigation. Several materials have been investigated, e.g. poly (·-hydroxy acids) such as polylactic acid (PLA) and polyglycolic acid (PGA). PLA and PGA are among the few synthetic degradable polymers that have been approved for human clinical use. Synthesized from D,D-L,L polylactic acid the BD 3D OPLA (Open- Cell Polylactic Acid) Scaffolds are available for basic research. This material has a facetted architecture, which is effective for culturing high density cell suspensions. 120 | bdbiosciences.com Scanning electron microscope image of a BD 3D OPLA Scaffold (Open-Cell Poly- Lactic Acid). BD 3D oPla ® scaffold applications include: • Short- and long-term growth and differentiation of a variety of cell types, including epithelial cells (e.g., hepatocytes), neurons, endothelialcells, osteoblasts, chondrocytes, fibroblasts, and smooth muscle cells • ECM pre-incubation for specific stem/progenitor cell differentiation assays • Dynamic cell seeding in larger vessels (e.g. BD Falcon 50 ml Conical Tubes) • Non-invasive evaluation of cell growth using the BD Oxygen Biosensor System
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204 Bioimaging SyStemS BD Bioscienc
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9 Pathway Bioimager Bioimaging SySt
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9 aCtone gPCR Screening assay Bioim
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10 Alphabetical Index AlphAbetIcAl
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10 numerical Index nUMeRIcAl Index
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BD Biosciences Worldwide Discovery
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