OPERATOR'S MANUAL BIOPROtect II Vent to ... - Baker Company

The Uses of the BIOPROtect II.II - PREPARING THE BIOPROtect II CABINET FOR USEThe BIOPROtect II cabinet has been designed to provide a work area that protects the experiment fromthe environment (Product protection), and protects the users from the experiment (personnel protection or‘containment’). The laminar flow biological safety cabinet is designed for work with Biosafety Levels 1, 2and 3 (low to moderate risk) agents as listed in The Center for Disease Control’s “Biosafety inMicrobiological and Biomedical Laboratories”, U.S. Department of Health and Human Services, PublicHealth Service, Centers for Disease Control and National Institutes of Health, U.S. Government PrintingOffice, Washington, D.C. 20402. HHS publication number (CDC) 84-8395.Extremely high risk agents assigned to Biosafety level 4 should never be used in this cabinet except in acertified BSL4 suit room. Please consult your safety professional for a proper risk assessment.Cautions* The use of any hazardous material in the cabinet requires that it be monitored by an industrial hygienist, safetyofficer or other qualified individual.* Explosive or flammable substances should never be used in the cabinet unless a qualified safety professionalhas evaluated the risk.* If hazardous biological work is to be performed, apply the appropriate biohazard decal which is enclosed. Thisis in accord with OSHA regulations, volume 39, number 125, Part II.* If chemical, radiological or other non-microbiological hazards are present, be sure to employ appropriateprotective measures in addition to formaldehyde decontamination. Call upon a suitably trained individual tomonitor the operation.Also refer to section 2 of this manual the section entitled “Decontamination”Location Within the LaboratoryPROPER PLACEMENT WITHIN THE LABORATORY IS ESSENTIAL.The ideal location for any laminar flow biological safety cabinet is in a dead-end corner of the laboratoryaway from personnel traffic, vents, doors, windows or any other sources of disruptive air currents. Publishedresearch from The Baker Company (see Rake ASM paper, reference #34 in Appendix) and unpublished testsperformed at the National Cancer Institute show that if a draft or other disruptive air current were to exceedthe intake velocity of the cabinet, then contamination can enter the work area or escape from it.Page 6 of 54