OPERATOR'S MANUAL BIOPROtect II Vent to ... - Baker Company

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DecontaminationWhenever maintenance, service or repairs are needed in a contaminated area of your cabinet, the unitmust first be decontaminated by an appropriate agent. The National Institute of Health, National CancerInstitute and the Center for Disease Control have all recommended the use of formaldehyde gas for mostmicrobiological agents. Its application requires individuals who are experienced in the decontamination ofcabinets, since the gas itself is toxic. Consult the NSF/ ANSI-standard-49-2002 Annex G for details andprocedures on the decontamination of Biological Safety cabinets.Whatever gas you choose, have the proper safety equipment (gas masks, protective clothing, etc.) withineasy reach. In addition, you will want to be sure that the gas you are using will be effective against all of thebiological agents within the cabinet. When you have decided which gas to use, post the antidote to it in avisible and nearby location.The volume of the BIOPROtect II cabinet is approximately 150 cubic feet, this will help you determinethe required amount of decontaminating gas.Carcinogens present a unique chemical deactivation problem and the standard biological decontaminationwill not be effective against chemicals or other non-biologic materials. With materials of this kind, consult aqualified safety professional.Warning! This procedure should be performed by qualified technicians only.Decontamination procedureSurface-disinfect the inside of the windows and all other surfaces on the viewscreen assemblys.Multiply the total volume of the cabinet (150 ft 3 ) by .3 gram/ft 3 ) to determine the amount ofparaformaldehyde required to decontaminate the cabinet. If the cabinet is vented to the outside you mustconsider the volume of the duct work in the paraformaldehyde calculation.Prior to sealing up the cabinet make sure all gas or flammable petcocks are closed and pressure tight.The inside cabinet work space must be at room temperature with 60% to 85% relative humidity. If relativehumidity is low (below 60%) add boiling water in the work space. Monitor the inside of the cabinet with ahumidity meter. Relatively humidity above 85% will require extra clean up which will be covered in step 15.Note:Without the proper relative humidity the formaldehyde gas will not be effective. The mode of entry offormaldehyde into the living organisms is through the cell wall by the absorption of water.Place a heating mantle with paraformaldehyde in the work space. The heating mantle must be able to reach450 degrees F and must have a grounded plug which should be plugged in to an outlet outside the cabinet.Place a second heating mantle in the cabinet with 60% more by weight of ammonium bicarbonate and 10%more if using ammonium carbonate, than paraformaldehyde. This will be used later in step 13 to neutralizethe formaldehyde gas.This step is recommended. Place spore strips inside the cabinet to confirm the decontamination process hasbeen successful.If the cabinet is vented to the room, use a sheet of plastic and seal the front access and exhaust port openings.These openings should be sealed such that the exhaust airflow recirculates back to the front access opening.This will promote the even distribution of formaldehyde gas throughout the cabinet.Page 13 of 54
If the cabinet is vented to the outside, then the exhaust transition should have a means to recirculate theexhaust airflow back to the cabinet blower if possible. This will promote the even distribution offormaldehyde gas through the exhaust filter. Seal the front access opening.Turn on the heating mantle containing the paraformaldehyde flakes.After 25% of the paraformaldehyde has depolymerized, turn on the cabinet blower for 10 to 15 seconds.Repeat after 50%, 75% and 100% of the paraformaldehyde has depolymerized, approx. 20 to 30 mins..Allow the formaldehyde gas a minimum residence time of 6 hours, preferably over night.Turn on the heating mantle containing ammonium bicarbonate (NH4HCO3). After 25% of the ammoniumbicarbonate has depolymerized turn on the cabinet blower for 10 to 15 seconds. Repeat after 50%, 75% and100% of the chemical has depolymerized to neutralize the formaldehyde. Turn on the cabinet blower andallow the two gases to circulate for at least one hour.Check for formaldehyde by using a gas sampling device (dregger tube) to be sure the formaldehyde is at asafe level inside the lab space.Cut open the plastic and vent the cabinet to the outside.Aseptically remove spore strip and place in Trypticase-soy broth and incubate for 7 days. No growth willverify the decontamination process.When cleaning up, you may find residual paraformaldehyde (white powder) on the metal or glass surfaces.To remove this, use ammonia in warm water, wear gloves and wipe down the affected surfaces. Theparaformaldehyde will dissolve in water and be neutralized by the ammonia.Page 14 of 54
Page 1 and 2: OPERATOR’S MANUALBIOPROtect IIVen
Page 3 and 4: Table of ContentsINTRODUCTION AND W
Page 5 and 6: BGA-BIOPROtect II-20 Canopy Exhaust
Page 7 and 8: Figure 1 Typical section with airfl
Page 9 and 10: SpecificationsDimensions:Outside: 1
Page 11 and 12: The Uses of the BIOPROtect II.II -
Page 13 and 14: Left Side & Right Side Outlet On /
Page 15 and 16: Set-up ProcedureThis section outlin
Page 17: Reacting to SpillsCaution: An Emerg
Page 21 and 22: IV - ON-SITE CHECKS AND MAINTENANCE
Page 23 and 24: The Airflow Balance! Warning !This
Page 25 and 26: Calibration procedure for Modus Air
Page 27 and 28: Maintenance NotesWARNING!Before any
Page 29 and 30: V. - TROUBLE SHOOTINGHere are some
Page 31 and 32: REPLACEMENT PARTS LISTPART NAMEQTYB
Page 33 and 34: Modus Installation and Operation Ha
Page 35 and 36: Page 30 of 54
Page 47 and 48: BGA-BIOPROtect II Vent to Room Page
Page 49 and 50: BGA-BIOPROtect II Vent to Room Page
Page 51 and 52: BGA-BIOPROtect II-19 Hard Exhaust C
Page 57 and 58: Wiring Diagram-Standard BIOPROtect
Page 59: WARRANTYThe Baker Company, Inc. exp

OPERATOR'S MANUAL BIOPROtect II Vent to ... - Baker Company

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