OPERATOR'S MANUAL BIOPROtect II Vent to ... - Baker Company

Start-up procedure1. If the unit has not been left running continuously, press the blower on/off switch. The switch will beilluminated when switched on. The cabinet’s audible alarm may activate for several seconds untilthe blowers have come up to full speed. Make sure that you have cabinet airflow, either by listeningfor blower sounds or feeling the airflow across your fingers. Check the reading on the air pressuregauge. The reading on the gauge should be consistent with the last time the unit was on.2. Turn on the fluorescent lights.The switch will become illuminated in the ‘on’ position and thefluorescent bulbs should activate.3. Wipe down the interior area of the cabinet with a surface disinfectant.NOTES:Some disinfectants may corrode or stain the steel surfaces. If this happens, clean thesurfaces afterward with a disinfectant-detergent and rinse with sterile tap water to preventcorrosion.Blocking the front and rear perforated grills must be avoided. If wipes or absorbenttowels are used on the work surface, be sure to keep them away from the grills.Check that the position of the rear wall dampers (three upper and three lower) are consistent with thesettings from the factory test report.After your equipment is in place inside the cabinet, close and secure the doors. All operations insidethe BIOPROtect II must be carried out through the unit’s 8” sash openings.Caution: The BIOPROtect II will not provide adequate personnel protection unless the front doors andviewscreens are closed.4. After a procedure has been completed, all equipment which has been in contact with the researchagent should be enclosed, and the entire surface decontaminated.Trays of discarded items should becovered. The cabinet should then be allowed to run for at least three minutes with no activity so thatthe airborne contaminant will be purged from the work area.5. After you have removed all materials, culture apparatus, etc., decontamination of the interiorsurfaces should be repeated.Check the work area carefully for spilled or splashed nutrient whichmight support bacterial growth. And never use the cabinet to store supplies or laboratory equipment.Page 11 of 54