Diffusion Processes with Hidden States from ... - FU Berlin, FB MI

A.2 Important Papers on the Rhodopsin-Transducin ProcessA.2 Important Papers on the Rhodopsin-Transducin ProcessThere are numerous papers, which are dealing with the analysis of the rhodopsin-transducin visualtransduction pathway (R-T-system):1. One of the early works on this topic is served by Michael J. Saxton and John C. Owicki in apaper from 1989 ([31]). Saxton and Owicki are investigating the question, how the concentrationof the numerous proteins affect the reaction rate of the rhodopsin-transducin-system.They are setting up a steady-state-diffusion model with concentration dependent diffusioncoefficients. They do this with the assumption of lateral diffusing transducin proteins on themembrane, and finally point out that there exists a maximum (optimal) reaction rate, andalong the way an optimal concentration. This conclusion grounds on the fact that on the onehand the reaction rate increases by the law of mass action, and on the other hand a higherconcentration would decrease the rate due to the decrease of the diffusion coefficients.2. A work from 1991 is [19], in which the authors deliver a model of transducin activationfrom its partial reactions, and solve the kinetic equations of the model both numerically, andfor small photo-activation, analytically.3. One year later, in the year 1992, a paper was published [6], in which the authors investigatethe activation of transducin by photo-excited rhodopsin in terms of the Michaelis-Mentenenzymologyand additionally by experiments.4. Lamb published his findings in a paper from 1996 [23], and in this work refers about asimulation of the diffusional interaction of the proteins by Monte Carlo techniques. Hisconclusion is that there exists a linear activation of many transducins by only one activatedrhodopsin.5. [15] is an experimental work from 1998, giving an account on the observation of transducinactivation with spatially and time resolved surface plasmon resonance.6. [11] deliver Master equation simulations of the diffusional protein interaction on a rectangular1µm 2 model membrane divided into 15 × 15 cells and with mono- and biomolecularreactions within cells and diffusional transitions between (neighboring) cells.101