ACCLAIM oa - Cromlab

IntroductionINTRODUCTIONAcclaim Columns Dedicated to HighQualityReliability and DurabilityAt Dionex we know that quality and reliability are essential toa successful analysis. Our columns are thoroughly tested individually,so that chromatographers can have full confidence in them.Manufacturing starts with an ultrapure silica substrate, using onlycarefully selected lots with narrow ranges of physical parameters.By design, the bonding processes are clean and repeatable with nounexpected changes in performance. Each batch of bonded silicareceives a full suite of validation tests appropriate to its intendeduse. The bonded silica is packed in precision-polished 316 stainlesssteel hardware using highly reliable processes. Each packedcolumn is tested to ensure the same great performance every time.The quality assurance reports for silica lot validation and columnperformance explain the test protocols, list the specifications, andshow the actual chromatograms.Base AsymmetryAU1.51.00.50Acclaim Columns Base Performance: AmitriptylineDCBA1234Amitriptyline Performance:# Acclaim Type EP Asym. N/mD 300 Å, 3 µm, C18 1.02 138KC 120 Å, 3 µm, C8 1.20 106KB 120 Å, 3 µm, PA C16 1.33 105KA 120 Å, 3 µm, C18 1.21 123KPeaks:1. Uracil2. Propranolol3. Toluene4. Doxepin55. Amitriptyline0 Minutes1220385Performance IndicatorsAcclaim bonded-silica columns have been designed to meetthe high quality standard needed in laboratories today. The innovativesurface chemistries deliver exceptional peak efficienciesfor a broad range of analytes. To ensure optimal performance, allDionex Acclaim products are thoroughly characterized using anumber of performance indicators, including surface coverage ofthe bonded phase, metal contamination, steric selectivity, columnpolarity, column hydrophobicity, and low silanol activity forbases. The specialty columns are also application-tested for theirspecific analysis, to ensure that each lot of bonded silica provideshigh-performance separations. The following charts and chromatogramsexplain how to interpret each of these tests.Good surface coverage of the silica gel with the bonded phase, and exhaustiveendcapping ensure that Acclaim columns provide symmetrical peaksand superior performance for the separation of basic drugs. Amitriptylineis a well-known example of a basic pharmaceutical that interacts stronglywith residual silanol groups on the silica gel, to produce asymmetricalpeaks.Acid AsymmetryPeak Asymmetry of AcidsPolarity IndexPolarity= [ K BBA · K’ phenol /K’ 2 toluene] × 100100806040201314202852535980AU01 231 23 4246Polar-EmbeddedLeading Brand E5 (As. = 3.49)5 (As. = 1.03)AcclaimPolarAdvantage64 6 8 10MinutesMobile Phase: 40/60 v/v 50 mM phosphate buffer,pH 3.25/CH 3 CNFlow Rate: 1.0 mL/minInj. Volume: 5 µLTemperature: 30 °CDetection: 254 nmPeaks: 1. Uracil2. Pyridine3. Phenol4. N,N-Dimethylaniline5. p-Butylbenzoic acid6. Toluene176540Acclaim C18Luna(2) C18Acclaim PASymmetryShield C18Discovery RP-Amide C16ProntoSIL EPS C18Acclaim PA2ZORBAX Bonus-RP20152Many polar-embedded columns perform well for a limited group of compounds.Acclaim PolarAdvantage columns use state of the art methodsof synthesis to eliminate unwanted secondary interactions. The result isoutstanding performance for hydrophilic and hydrophobic acidic, basic,and neutral analytes.The polarity index chart above ranks commercially available stationaryphases according to their polarity relative to one another, under the sameconditions. The more similar the polarity index of two stationary phases,the more similar their separation of polar compounds should be. If onecolumn does not provide adequate resolution, choosing a column with adifferent polarity index may deliver a better separation.- 10 -www.dionex.com