ACCLAIM oa - Cromlab

Acclaim PolarAdvantage IIAU12132Dewetting Test in 100% Aqueous EluentColumns: 5 µm, 4.6 x 150 mmMobile Phase: 2.5 mM methanesulfonic acidTemperature: 30 °CFlow Rate: 1.0 mL/minConventional C18Before stop-flow testAfter stop-flow test3AUInj. Volume: 5 µLDetection: UV, 254 nmPeaks:111. Cytosine2. Uracil3. Thymine22Acclaim PA2Before stop-flow test3After stop-flow test3% Remaining Retention11010090807060Accelerated Stability Test at Low pHCompetitor CCompetitor ACompetitor BAcclaim PA2500 10 20 30 40Time of Purge (Days)Column: See chart, 5 µmDimensions: 4.6 × 150 mmMobile Phase: CH 3 CN/1% TFA*,pH 1 v/v 50/50Temperature: 50 °CFlow Rate: 1.0 mL/minInj. Volume: 5 µLDetection: UV, 254 nmAnalyte: Toluene*Trifluoroacetic acid201420 2 4 6 8 10MinutesHydrolytic Stability0 2 4 6 8 10MinutesDespite their many advantages, the hydrolytic stability ofpolar embedded phases is often inferior to conventional C18 phasesas a result of lower ligand coverage and the hydrophilic natureof the embedded group. The Acclaim PA2 has been designed toprovide all the advantages of polar embedded phases, but withenhanced stability at both low and high pH.HPLC separations of polar analytes are often run at low pH(PH