ACCLAIM oa - Cromlab

Acclaim SurfactantGuaranteed Performance for SurfactantsThe exceptional performance of Acclaim Surfactant columnsfor the separation of anionic, cationic and non-ionic surfactantsis confirmed with every new lot of bonded silica that is manufactured.In addition each column is individually tested with a simplemixture of anionic, cationic and non-ionic probes, to ensure thatevery column is packed consistently and well.Performance SpecificationsColumn Efficiency (N/m) 76,500Asymmetry (EP) 0.95–1.32Retention time 7.50–8.60Example chromatographic specifications for Acclaim Surfactant4.6 × 150 mm. Please refer to Part 4 for a detailed description of thetests and specifications. Specifications are subject to change at thediscretion of Dionex.900LOT VALIDATION #6 #1, SFC_P01 (isocratic)Vlittle or no modifications, because both detectors share the samemobile phase requirements. Conductivity detection of cationic surfactantscan also be used. Because of the versatility of this column,methods using all these detectors have been developed and can befound in this catalog, in the Acclaim product information bulletin,or on the Dionex website.AbundanceAnalysis of POE (1) Lauryl Sulfate (LC-ESI-MS)12:712:812:912:512:612:412:212:312:012:1 (alkyl chain: n)14:2Column: Acclaim Surfactant, 5 µmDimension: 4.6 × 150 mmMobile Phase: CH 3 CN/0.1 M NH 4 OAc,pH 5.4 v/v 60/40Temperature: 25 °CFlow Rate: 1 mL/min (0.2 mL/min to MS)Inj. Volume: 25 µLDetection: MS (ESI negative)Sample: POE (1) lauryl sulfate (100 ppm)14:014:1n ~ 1OSodium POE (1) Lauryl SulfateO(OCH 2 CH 2 ) n - O-S-O Na1 23 450050 10 20 30 40Minutes22795-100min0.0 10.0 20.0 30.0Chromatogram of a group of surfactants, taken from the certificate forlot #013-05-002. Dionex monitors and controls the selectivity of cationic,nonionic and anionic surfactants using the recommended standard conditionsfor this column.Detector CompatibilityAlthough UV detection is the most commonly used of HPLCdetectors, not all surfactants possess chromophores. This problemcan be overcome by the use of a universal detector, such asevaporative light-scattering detection (ELSD) or refractive index(RI) detection. ELSD is compatible with gradient methods and isfar more sensitive than RI. In addition, methods developed withELSD can be easily transferred to LC-ESI-MS applications withAUDimensions: 4.6 × 150 mmMobile Phases: Acetonitrile/100 mMNH4OAc, pH 5.4,v/v 70/30 for Acclaim SurfactantAcclaim SurfactantC11-LASv/v 50/50 for Acclaim PAC10-LASTemperature: 30 °CC12-LASFlow Rate: 1 mL/minInj. Volume: 5 µLC13-LAS Detection: UV, 225 nmC11-LASC10-LASC12-LASAcclaim PAHC13-LASCH 3 (CH 2 ) n C (CH 2 ) m CH 30 5Analyis of Sodium Dodecylbenzene Sulfonate (LAS)10 15 20 25MinutesSO 3 Nam + n =7-10Sodium Dodecylbenzene Sulfonate21078SURFACTANTOrdering Information: Acclaim SurfactantStandard particle size is nominally 5-mm. Analytical columns are stocked in 4.6–mm-diameter widths and a couple of lengths. Guard cartridgesin 4.3-mm sizes, packed with 5-mm particles are recommended to protect the analytical columns. Other geometries can be made toorder. Enquire with your Dionex sales representative.Please order through your local Dionex office or distributor. Refer to the following part numbers.Acclaim Surfactant Analytical Columns5 µm 4.6 × 150 mm ............................... 0632015 µm 4.6 × 250 mm ............................... 063203Acclaim Surfactant Guard Columns5 µm 4.3 × 10 mm (pack of 2) . .....................063215Biocompatible guard cartridge holder ................059456Guard for analytical column coupler. ..................059457Acclaim holder and coupler kit .......................059526These columns are designed for optimal performance using Dionex UltiMate 3000 and ICS-3000 chromatography instruments.www.dionex.com - 25 -