ACCLAIM oa - Cromlab

IntroductionMetal Contamination400300Column: Acclaim 300 C18, 3 µmDimensions: 4.6 × 150 mmMobile Phase: 50/50 v/v water/methanolTemperature: 30 °CFlow Rate: 1.0 mL/minRA5.75Evaluation of Metal Contamination1Inj. Volume: 10 µLDetection: 214 nmSamples: 1. 4,4'-bipyridyl 250 ng/µL2. 2,2'-bipyridyl 10002Competitor A, 5 µmSteric SelectivityComparison of Steric Selectivity forAcclaim 120 C18 and Acclaim PAAcclaim 120 C182 3 42 34Competitor 1AU11Competitor 212 3 4Columns: As indicatedMobile Phase: 90% methanolTemperature: 30 °CFlow Rate: 1 mL/minInj. Volume: 5 µLPeaks: 1. Toluene2. Phenanthrene3. o-Terphenyl4. TriphenyleneINTRODUCTION200mAU10002.121.445.154.481.29Competitor B, 5 µmCompetitor C, 3 µmCompetitor D, 5 µmCompetitor E, 3 µmAcclaim 300 C18, 3 µm–500 1 2 3 4 5 6MinutesNote: Ratio of asymmetries (RA) is quoted as the asymmetry of Peak 2 divided by Peak 1.Metal contamination can interfere with the separation of many differentsubstances, resulting in peak tailing and concomitant poor peakresolution. 2,2’-bipyridyl is a strong chelating agent, and will revealany contamination by metal ions. The figure above shows that Acclaimoutperforms five competitor columns in this test.17590AU0 2 4 6 8 10Minutes12, 34Competitor 3Competitor 21 2 3 4112 3 4Competitor 12 3 Acclaim PolarAdvantage40 2 4 6 8 10 12MinutesPeaks:1. Toluene 2. Phenanthrene 3. o–Terphenyl 4. TriphenyleneMe17037Column: 5 µmDimensions: 150 × 4.6 mmMobile Phase: 90/10 v/v MeOH/D.I. H 2OTemperature: 30 °CFlow Rate: 1.0 mL/minInj. Volume: 5 µLDetection: UV, 254 nmDepending on the bonding chemistry, various C18 columns can havedifferent selectivity to molecular shape. Acclaim may be just what youneed for your unique separation challenge.19309www.dionex.com - 11 -