ACCLAIM oa - Cromlab

Ch e m i c a l ApplicationsSurfactantsmVSeparation of Cationic Surfactants Using ELSD1230 10 2030Minutes45Column: Acclaim Surfactant, 5 µmDimensions: 4.6 × 150 mmMobile Phase: (A) Acetonitrile(B) 0.1 M NH 4 OAc, pH 5.4Gradient: 25–85% A in 30 minTemperature: 30 °CFlow Rate: 1 mL/minInj. Volume: 25 µLDetection: ELSDPeaks:1. Lauryl pyridinium chloride2. Lauryldimethylbenzyl ammoniumchloride3. Octylphenoxyethoxyethyldimethylbenzyl ammonium chloride4. Cetyltrimethylammonium chloride5. Cetylpyridinium chloride21082mVUV, 225 nmELSDAnalysis of a ShampooLaureth Sulfate0 5 10 15 20 25 30 35 40 45MinutesColumn: Acclaim Surfactant, 5 µmDimensions: 4.6 × 150 mmMobile Phase: (A) Acetonitrile(B) 0.1 M NH 4 OAc, pH 5.4Gradient: 25–80% A in 30 min,then hold at 80% A for 15 minTemperature: 30 °CFlow Rate: 1 mL/minInj. Volume: 10 µLDetection: ELSD, UV21083Analysis of a MouthwashAnalysis of a Fabric SoftenermV123Column: Acclaim Surfactant, 5 µmDimensions: 4.6 × 150 mmMobile Phase: (A) Acetonitrile(B) 0.1 M NH 4 OAc, pH 5.4Gradient: 25–80% A in 30 minTemperature: 30 °CFlow Rate: 1 mL/minInj. Volume: 20 µL (direct injection)Detection: ELSDPeaks: 1. Sodium sacchrin and benzoate2. Domiphen bromide3. Cetylpyridinium chloridemVColumn: Acclaim Surfactant, 5 µmDimensions: 4.6 × 150 mmMobile Phase: (A) Acetonitrile(B) THF(C) 0.1 M NH 4 OAc, pH 5.4Gradient: 40% A/10% B/50% C to 75% A/10% B/75% C in 20 min,then hold for 10 minTemperature: 30 °CFlow Rate: 1 mL/minInj. Volume: 20 µLDetection: ELSDPeaks: Methyl bis[ethyl(tallowate)]-2-hydroxyethyl ammonium methylsulfate (500×)010 2030Minutes21120010 2030Minutes21121Analysis of ZONYL FSO FluorosurfactantSeparation of Ethoxylated QuatsmVColumn: Acclaim Surfactant, 5 µmDimensions: 4.6 × 150 mmMobile Phase: (A) Acetonitrile,(B) H 2 OGradient: 40–70% A in 30 min,then hold at 70% A for 5 minFlow Rate: 1 mL/minInj. Volume: 10 µLDetection: ELSDR f CH 2 CH 2 O(CH 2 CH 2 O) x HmVColumn: Acclaim Surfactant, 5 µmDimensions: 4.6 × 150 mmMobile Phase: Acetonitrile/0.2 M NH 4 OAc,pH 5.4 v/v 40/60Temperature: 30 °CFlow Rate: 1 mL/minInj. Volume: 25 µLDetection: ELSDSample: Ethoquad 18/25 (500×)R f = F(CF 2 CF 2 ) yx = 0 to about 15y = 1 to about 7Zonyl FSO fluorosurfactantAPPLICATIONS0 10 20 30 35Minutes210810 5 10 15 20 25 30 35 40Minutes21116- 42 -www.dionex.com